Human GPC3/Glypican 3 Sandwich ELISA Kit-qPCR

CAT#: TM-HMM101
Product Type: Sandwich ELISA kit
Target: GPC3
Reactivity: Human
Applications: GPC3 detection
Description
This product is an innovative new assay that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR.
Features
High sensitivity qPCR readout for the quantitative measurement of human Glypican 3 in serum, plasma, and cell culture supernatants.
Applications
GPC3 detection
Target
GPC3
Reactivity
Human
Detection Method
qPCR
Method Type
Quantitative
Sample Type
Cell culture supernatants, plasma, serum
Size
96-well microplate
Receptor Family
Glypican-related integral membrane proteoglycan family (GRIPS)
Qualified With
Certificate of quality
Components
Glypican 3 microplate; Wash buffer; Standards; Assay diluent A; Assay diluent B; Detection affinity reagent for glypican 3; Detection reagent; Primer solution; PCR master mix; PCR preparation buffer; Final wash buffer
Sensitivity
0.08 ng/mL
Plate
1, 2, or 5
Reagent Preparation
Real-time PCR instrument, Bio-Rad recommended; Precision pipettes to deliver 2 µL to 1 mL volumes; Adjustable 1-25 mL pipettes for reagent preparation; 100 mL and 1 liter graduated cylinders; Absorbent paper; Distilled or deionized water; Log-log graph paper or computer and software for data analysis; Tubes to prepare standard or sample dilutions; Heating block or water bath capable of 80°C
Assay Procedure
Prepare all reagents, samples and standards as instructed; Add 25µL standard or sample to each well. Incubate for 2.5 hours at room temperature or overnight at 4°C; Add 25 µL detection affinity reagent to each well; Incubate 1 hour at room temperature; Add 25µL of Detection Reagent to each well; Incubate 1 hour; Add 15µL Primer solution 10µL of PCR master mix to each well; Run real-time PCR
Target/Description
Glypican-3 is a protein that, in humans, is encoded by the GPC3 gene.
Accession Number
P51654
Storage
Stored for up to 6 months at 2° to 8°C from the date of shipment. Standard (recombinant protein) should be stored at -20°C or -80°C (recommended at -80°C) after reconstitution.
Restrictions
For research use only. Intended for use by laboratory professionals.
Alternative Name
GPC; DGSX; Glypican proteoglycan 3; Glypican 3; Glypican-3; GTR2-2; Intestinal protein OCI-5; SGB; SGBS1; SGBS; MXR7; OCI-5; OCI5; SDYS; Secreted glypican-3; Heparan sulphate proteoglycan
Synonyms
GPC; DGSX; Glypican proteoglycan 3; Glypican 3; Glypican-3; GTR2-2; Intestinal protein OCI-5; SGB; SGBS1; SGBS; MXR7; OCI-5; OCI5; SDYS; Secreted glypican-3; Heparan sulphate proteoglycan
Background
Glypican-3 is a protein that, in humans, is encoded by the GPC24 gene
Gene ID
2719
For Research Use Only | Not For Clinical Use
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