Human IDO1 Recombinant Cell Line, HEK293 (CAT#: IOC-LX080)

Creative Biolabs has developed several immune checkpoint stable cell Lines expressing the most popular immune checkpoints to accelerate clients' immunotherapy discovery and clinical translation. Stable Recombinant HEK293 IDO1 cell line expressing tetracycline-inducible human indoleamine 2,3-dioxygenase (IDO1) Genbank accession number NM_002164. Which can be used in binding assays and functional assay.

Stable cell lines for increased reliability and reproducibility;
Protein expression at the cell surface validated by FACS or WB;

Monitor IDO1 pathway activity. Screen for activators or inhibitors of IDO1 in a cellular context.

Human

HEK293

IDO1

L-tryptophan (L-Trp) is an essential amino acid necessary for protein synthesis in mammalian cells and the L-Trp to kynurenine (Kyn) pathway is firmly established as a key regulator of innate and adaptive immunity. Catabolism of L-Trp to Kyn maintains an immunosuppressive microenvironment by starving immune cells of L-Trp and releasing degradation products of L-Trp that have immunosuppressive functions. Indoleamine 2,3- dioxygenases (IDO1 & IDO2), two of the rate limiting enzymes in this pathway, are upregulated in many tumors, providing cancer cells with an avenue for immune evasion.