Short Decsription
The FluoroSpot assay is an inventive development of the ELISpot assay and uses fluorescent detection instead of colorimetric.
Description
The human IFN-γ/IL-10/Granzyme B FluoroSpot kit is perfect for users who want a convenient analysis with minimal analysis variability. It can count cells that secrete one, two or three analytes. The kit includes a low-fluorescence plate pre-coated with a monoclonal capture antibody, a monoclonal detection antibody, a secondary reagent coupled to a fluorophore, a fluorescence enhancer, and an anti-cd28 monoclonal antibody for costimulation. Human T cell polyclonal activator (mAb CD3-2) was used as a positive control for cytokine secretion. The pre-coated plate enables faster determination and minimal variability.
Applications
FluoroSpot
Comment
Plates can be washed using a multi-channel micropipette. A regular plate washer can be used in washing steps when sterile conditions are not required, provided that the washing head is adapted to ELISpot/FluoroSpot plates.
Target
Granzyme B, IFNG, IL10
Reactivity
human
Detection Method
Sandwich
Method Type
Sandwich
Sample Type
cell suspension
Specificity
Recognizes natural human Granzyme B, IFNG, IL10

For Research Use Only | Not For Clinical Use

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