Human IFNG ELISA Kit, Lot 21AO-986 [Cancer Immune Checkpoint Assay Kit]

The kit is designed for in vitro quantitative measurement of Human IFNG in Cell Culture Supernatant, Plasma, Serum.

Human Immunoquantitative (PCR-Based) IFN-Gamma ELISA Kit for cell culture supernatants, plasma, and serum samples.

ELISA

The Immuno-Quantitative ELISA kits are an innovative assay platform that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR. This results in an assay that is simultaneously familiar and cutting edge and enables the use of only 1/10th the sample volume while also providing 10x more sensitivity than a traditional ELISA.

Excellent for follow up/confirmation of antibody array data.
Pre-coated 96 well plate.
Strip plate format minimizes waste.
Complete kit includes all necessary reagents.
10x more sensitive than ELISA.
Reproducible and reliable.
Optimized for serum, plasma, urine and cell culture medium.
1/10th the sample volume of an ELISA.

IFNG

Human

qPCR

Sandwich ELISA

Semi-Quantitative

Cell Culture Supernatant, Plasma, Serum

IFN-Gamma Microplate (Item A): 96 well PCR plate coated with anti-human IFN-Gamma.
Wash Buffer I Concentrate (20x) (Item B): 20x concentrated solution.
Standards (Item C): recombinant human IFN-Gamma.
Assay Diluent A (Item D): diluent buffer, 0.09% sodium azide as preservative.
Assay Diluent B (Item E): 5x concentrated buffer.
Detection Affinity Reagent for IFN-Gamma (Item F): 4x concentrated solution of anti-human IFN-Gamma affinity reagent.
Primer Solution (Item G).
PCR Master Mix (Item H).
PCR Preparation buffer (Item I): 10x concentrated buffer.
Final Wash Buffer (Item J): 10x concentrated buffer.

Real-time PCR instrument, Bio-Rad recommended.
Precision pipettes to deliver 2 μL to 1 mL volumes.
Adjustable 1-25 mL pipettes for reagent preparation.
100 mL and 1 liter graduated cylinders.
Absorbent paper.
Distilled or deionized water.
Log-log graph paper or computer and software for data analysis.
Tubes to prepare standard or sample dilutions.
Heating block or water bath capable of 80°C.

3 pg/mL

Pre-coated

Serum spike tests show recovery is 94.3% with a range of 88.4 to 102.1%.

4 °C,-20 °C,-80 °C

May be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standard (recombinant protein) should be stored at -20°C or -80°C (recommended at -80°C) after reconstitution. Opened PCR plate or reagents may be stored for up to 1 month at 2°C to 8.

6 months

May be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standard (recombinant protein) should be stored at -20°C or -80°C (recommended at -80°C) after reconstitution. Opened PCR plate or reagents may be stored for up to 1 month at 2° to 8.

For Research Use only

Interferon gamma

IFG; IFI; IFN-g; Ifg; IFNG2; IFN-gamma; IFN-G; IFNG; IFNgamma; TCRalpha; INF-G; ifng; interferon gamma; interferon; gamma 1-2; IFNG; Ifng; ifng1-2

Synonyms: Interferon gamma (IFN-gamma) (Immune interferon), IFNG

3458

P01579

Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy

1.Prepare all reagents, samples and standards as instructed.
2.Add 25 μL standard or sample to each well. Incubate for 2.5 hours at room temperature or overnight at 4°C.
3.Add 25 μL detection affinity reagent to each well. Incubate 1 hour at room temperature.
4.Add 25μL of quantitative ELISA Detection Reagent to each well. Incubate 1 hour.
5.Add 15μL Primer solution 10μL of PCR master mix to each well.
6.Run real-time PCR.