Human IL-13 (IL13) ELISA Kit, Lot 21AO-525 [Cancer Immune Checkpoint Assay Kit]

The kit is designed for in vitro quantitative measurement of Human IL-13 (IL13) in Cell Culture Supernatant, Plasma, Serum.

Human Immunoquantitative (PCR-Based) IL-13 ELISA Kit for cell culture supernatants, plasma, and serum samples.

ELISA

The Immuno-Quantitative ELISA kits are an innovative assay platform that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR. This results in an assay that is simultaneously familiar and cutting edge and enables the use of only 1/10th the sample volume while also providing 10x more sensitivity than a traditional ELISA.

Excellent for follow up/confirmation of antibody array data.
Pre-coated 96 well plate.
Strip plate format minimizes waste.
Complete kit includes all necessary reagents.
10x more sensitive than ELISA.
Reproducible and reliable.
Optimized for serum, plasma, urine and cell culture medium.
1/10th the sample volume of an ELISA.

IL-13 (IL13)

Human

qPCR

Sandwich ELISA

Semi-Quantitative

Cell Culture Supernatant, Plasma, Serum

IL-13 Microplate (Item A): 96 well PCR plate coated with anti-Human IL-13.
Wash Buffer I Concentrate (20x) (Item B): 25 ml of 20x concentrated solution.
Standards (Item C): 2 vials of recombinant Human IL-13.
Assay Diluent A (Item D): 30 ml diluent buffer, 0.09% sodium azide as preservative.
Assay Diluent B (Item E): 15 ml of 5x concentrated buffer.
Detection Affinity Reagent for IL-13 (Item F): 2 vials of a 4x concentrated solution of anti-Human IL-13 affinity reagent.
Quantitative ELISA Detection Reagent (Item G): 1.4ml of a 10x concentrated stock.
Primer Solution (Item I): 1.7 ml vial.
PCR Master Mix (Item J): 1.2 ml vial.
PCR Preparation buffer (Item K): 1ml vial of 10x concentrated buffer.
Final Wash Buffer (Item L): 10ml vial of 10x concentrated buffer

Real-time PCR instrument, Bio-Rad recommended.
Precision pipettes to deliver 2 μL to 1 mL volumes.
Adjustable 1-25 mL pipettes for reagent preparation.
100 mL and 1 liter graduated cylinders.
Absorbent paper.
Distilled or deionized water.
Log-log graph paper or computer and software for data analysis.
Tubes to prepare standard or sample dilutions.
Heating block or water bath capable of 80°C.

Pre-coated

Recommended Dilution for serum and plasma samples2-10 fold

-20 °C

May be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standard (recombinant protein) should be stored at -20°C or -80°C (recommended at -80°C) after reconstitution. Opened PCR plate or reagents may be stored for up to 1 month at 2°C to 8°C. Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.

6 months

May be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standard (recombinant protein) should be stored at -20°C or -80°C (recommended at -80°C) after reconstitution. Opened PCR plate or reagents may be stored for up to 1 month at 2°C to 8°C. Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.

For Research Use only

Interleukin 13

IL13; IL-13; P600; Il-13; L13; interleukin 13; IL13; Il13

3596

P35225

JAK-STAT Signaling, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport

1.Prepare all reagents, samples and standards as instructed.
2.Add 25 μL standard or sample to each well. Incubate for 2.5 hours at room temperature or overnight at 4°C.
3.Add 25 μL detection affinity reagent to each well. Incubate 1 hour at room temperature.
4.Add 25μL of quantitative ELISA Detection Reagent to each well. Incubate 1 hour.
5.Add 15μL Primer solution 10μL of PCR master mix to each well.
6.Run real-time PCR.