Human IL 18 ELISA Kit, Lot 21AO-1314 [Cancer Immune Checkpoint Assay Kit]

The kit is designed for in vitro quantitative measurement of Human IL 18 in Cell Culture Supernatant, Plasma, Serum.

Human Immunoquantitative (PCR-Based) IL-18 ELISA Kit for cell culture supernatants, plasma, and serum samples.

ELISA

The Immuno-Quantitative ELISA kits are an innovative assay platform that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR. This results in an assay that is simultaneously familiar and cutting edge and enables the use of only 1/10th the sample volume while also providing 10x more sensitivity than a traditional ELISA.

Detection of quantitative protein levels in biological fluids.
Validation of antibody array results.
Validation of biomarker discovery studies.

IL 18

Human

qPCR

DNA-Binding ELISA

Quantitative

Cell Culture Supernatant, Plasma, Serum

IL-18 R alpha (IL-1 R5) Microplate (Item A): 96 well PCR plate coated with anti-Human IL-18 R alpha (IL-1 R5).
Wash Buffer I Concentrate (20x) (Item B): 25 ml of 20x concentrated solution.
Standards (Item C): 2 vials of recombinant Human IL-18 R alpha (IL-1 R5).
Assay Diluent A (Item D): 30 ml diluent buffer, 0.09% sodium azide as preservative.
Assay Diluent B (Item E): 15 ml of 5x concentrated buffer.
Detection Affinity Reagent for IL-18 R alpha (IL-1 R5) (Item F): 2 vials of a 4x concentrated solution of anti-Human IL-18 R alpha (IL-1 R5) affinity reagent.
Quantitative ELISA Detection Reagent (Item G): 1.4ml of a 10x concentrated stock.
Primer Solution (Item I): 1.7 ml vial.
PCR Master Mix (Item J): 1.2 ml vial.
PCR Preparation buffer (Item K): 1ml vial of 10x concentrated buffer.
Final Wash Buffer (Item L): 10ml vial of 10x concentrated buffer.

Real-time PCR instrument, Bio-Rad recommended.
Precision pipettes to deliver 2 μL to 1 mL volumes.
Adjustable 1-25 mL pipettes for reagent preparation.
100 mL and 1 liter graduated cylinders.
Absorbent paper.
Distilled or deionized water.
Log-log graph paper or computer and software for data analysis.
Tubes to prepare standard or sample dilutions.
Heating block or water bath capable of 80°C.

0.0048 pg/mL

Pre-coated

4 °C,-20 °C,-80 °C

May be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standard (recombinant protein) should be stored at -20°C or -80°C (recommended at -80°C) after reconstitution. Opened PCR plate or reagents may be stored for up to 1 month at 2°C to 8°C.

6 months

May be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standard (recombinant protein) should be stored at -20°C or -80°C (recommended at -80°C) after reconstitution. Opened PCR plate or reagents may be stored for up to 1 month at 2°C to 8°C.

For Research Use only

Interleukin 18 (IL-18)

Igif; Il-18; IL-18; IGIF; ChIL-18; IL-1g; IL1F4; IL18; interleukin 18; Il18; IL18

8809

Q13478

Cellular Response to Molecule of Bacterial Origin, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome

1.Prepare all reagents, samples and standards as instructed.
2.Add 25 μL standard or sample to each well. Incubate for 2.5 hours at room temperature or overnight at 4°C.
3.Add 25 μL detection affinity reagent to each well. Incubate 1 hour at room temperature.
4.Add 25μL of quantitative ELISA Detection Reagent to each well. Incubate 1 hour.
5.Add 15μL Primer solution 10μL of PCR master mix to each well.
6.Run real-time PCR.