Human IL-4 (IL4) ELISA Kit, Lot 21AO-480 [Cancer Immune Checkpoint Assay Kit]

CAT#: IOK-05-P391
Product Type: ELISA Kit
Target: IL-4 (IL4)
Short Description
The kit is designed for in vitro quantitative measurement of Human IL-4 (IL4) in Cell Culture Supernatant, Saliva, Serum, Tissue Homogenate.
Description
For the quantitative determination of human interleukin 4 (IL-4) concentrations in serum, tissue homogenates, cell culture supernates, saliva.
Applications
ELISA
Application Notes
Optimal working dilution should be determined by the investigator.
Target
IL-4 (IL4)
Reactivity
Human
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Analytical Method
Quantitative
Sample Type
Cell Culture Supernatant, Saliva, Serum, Tissue Homogenate
Specificity
This assay has high sensitivity and excellent specificity for detection of human IL-4. No significant cross-reactivity or interference between human IL-4 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between human IL-4 and all the analogues, therefore, cross reaction may still exist.
Components
Assay plate
Standard
HRP-avidin (100 x concentrate)
Biotin-antibody (100 x concentrate)
Sample Diluent
HRP-avidin Diluent
Biotin-antibody Diluent
Wash Buffer (25 x concentrate)
TMB Substrate
Stop Solution
Adhesive Strip
Sensitivity
1.56 pg/mL
Sample Volume
100 μL
Assay Time
1 - 4.5 h
Plate
Pre-coated
Reagent Preparation
Biotin-antibody (1×) - Centrifuge the vial before opening.
HRP-avidin (1×) - Centrifuge the vial before opening.
Wash Buffer (1×) - If crystals have formed in the concentrate, warm up to room temperature and mix gently until the crystals have completely dissolved.
Standard - Centrifuge the standard vial at 6000-10000rpm for 30s.
Assay Precision
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess.
Storage
4 °C,-20 °C
Storage Comment
Unopened kit Store at 2 - 8°C. May be stored for up to 1 month at 2 - 8°C. Coated assay Try to keep it in a sealed aluminum foil bag, plate and avoid the damp. Standard May be stored for up to 1 month at 2 - 8°C. If Biotin-antibody doesn't make recent use, better keep it store at HRP-avidin -20°C. Biotin-antibody Diluent Opened kit HRP-avidin Diluent Sample May be stored for up to 1 month at 2 - 8°C.
Expiry Date
6 months
Note
Unopened kit Store at 2 - 8°C. May be stored for up to 1 month at 2 - 8°C. Coated assay Try to keep it in a sealed aluminum foil bag, plate and avoid the damp. Standard May be stored for up to 1 month at 2 - 8° C. If Biotin-antibody don't make recent use, better keep it store at HRP-avidin -20°C. Biotin-antibody Diluent Opened kit HRP-avidin Diluent Sample May be stored for up to 1 month at 2 - 8°C.
Restrictions
For Research Use only
Alternative Name
interleukin 4
Synonyms
IL4; IL-4; BCGF-1; BCGF1; BSF-1; BSF1; Il-4; Il4e12; IL2; interleukin 4; IL4; Il4
Background
Abbreviation: IL4
Alias: BCGF-1, BCGF1, BSF1, IL-4, MGC79402, B cell growth factor 1|B_cell stimulatory factor 1|lymphocyte stimulatory factor 1
Gene ID
3565
UniProt
P05112
Pathways
JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation
For Research Use Only | Not For Clinical Use
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