Human IL1A ELISA Kit, Lot 21AO-300 [Cancer Immune Checkpoint Assay Kit]

The kit is designed for in vitro quantitative measurement of Human IL1A in Cell Culture Supernatant, Plasma (EDTA - heparin - citrate), Serum.

Sandwich ELISA for Quantitative Detection of Antigen

ELISA

Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 300pg/mL, 150pg/mL, 75pg/mL, 37.5pg/mL, 18.8pg/mL, 9.4pg/mL, 4.7pg/mL human IL-1 alpha standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well.
Blood Product Anticoagulant: Heparin Sodium.
ELISA Dilution: 4.7pg/mL-300pg/mL.

IL1A

Human

Colorimetric

Sandwich ELISA

Quantitative

Cell Culture Supernatant, Plasma (EDTA - heparin - citrate), Serum

100 μL

Pre-coated

RT,4 °C,-20 °C

Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

For Research Use only

Interleukin-1-alpha (IL-1-alpha)

IL-1A; IL1; IL1-ALPHA; IL1F1; Il-1a; IL-1 alpha; IL-1alpha; L1A; interleukin 1 alpha; IL1A; Il1a

3552

P01583

NF-kappaB Signaling, Autophagy, Cancer Immune Checkpoints