Human IL1B ELISA Kit, Lot 21AO-259 [Cancer Immune Checkpoint Assay Kit]

The kit is designed for in vitro quantitative measurement of Human IL1B in Cell Culture Supernatant, Cerebrospinal Fluid, Serum, Urine.

For the quantitative determination of human interleukin 1β (IL-1β) concentrations in serum, cell culture supernates, urine, cerebrospinal fluid (CSF).

ELISA

Optimal working dilution should be determined by the investigator.

IL1B

Human

Colorimetric

Sandwich ELISA

Quantitative

Cell Culture Supernatant, Cerebrospinal Fluid, Serum, Urine

This assay has high sensitivity and excellent specificity for detection of human IL-1β. No significant cross-reactivity or interference between human IL-1β and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between human IL-1β and all the analogues, therefore, cross reaction may still exist.

Assay plate
Standard
HRP-avidin (100 x concentrate)
Biotin-antibody (100 x concentrate)
Sample Diluent
HRP-avidin Diluent
Biotin-antibody Diluent
Wash Buffer (25 x concentrate)
TMB Substrate
Stop Solution
Adhesive Strip

31.25 pg/mL

100 μL

1 - 4.5 h

Pre-coated

Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess.

4 °C,-20 °C

Unopened kit Store at 2 - 8°C. Do not use the kit beyond the expiration date May be stored for up to 1 month at 2 - 8°C. Coated assay Try to keep it in a sealed aluminum foil bag, plate and avoid the damp. Standard May be stored for up to 1 month at 2 - 8°C. If Biotin-antibody doesn't make recent use, better keep it store at HRP-avidin -20°C. Biotin-antibody Diluent Opened kit HRP-avidin Diluent Sample May be stored for up to 1 month at 2 - 8°C. Diluent Wash Buffer TMB Substrate Stop Solution *Provided this is within the expiration date of the kit.

6 months

Unopened kit Store at 2 - 8°C. Do not use the kit beyond the expiration date May be stored for up to 1 month at 2 - 8°C. Coated assay Try to keep it in a sealed aluminum foil bag, plate and avoid the damp. Standard May be stored for up to 1 month at 2 - 8° C. If Biotin-antibody don't make recent use, better keep it store at HRP-avidin -20°C. Biotin-antibody Diluent Opened kit HRP-avidin Diluent Sample May be stored for up to 1 month at 2 - 8°C. Diluent Wash Buffer TMB Substrate Stop Solution *Provided this is within the expiration date of the kit.

For Research Use only

Interleukin 1 Beta (IL1b)

IL-1; IL1-BETA; IL1F2; IL-1BETA; IL1beta; il1-b; zgc:111873; IL-1B; IL-1beta; Il-1b; IL1B; IL-1 beta; IL-1b; interleukin 1 beta; interleukin 1; beta; IL1B; il1b; Il1b

Abbreviation: IL1B
Alias: IL-1, IL1-BETA, IL1F2, catabolin|preinterleukin 1 beta|pro-interleukin-1-beta

3553

P01584

NF-kappaB Signaling, Interferon-gamma Pathway, TLR Signaling, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagy, Cancer Immune Checkpoints, Inflammasome

Biotin-antibody (1×) - Centrifuge the vial before opening.
HRP-avidin (1×) - Centrifuge the vial before opening.
Wash Buffer (1×) - If crystals have formed in the concentrate, warm up to room temperature and mix gently until the crystals have completely dissolved.
Standard - Centrifuge the standard vial at 6000-10000rpm for 30s.