Human IL1B ELISA Kit, Lot 21AO-263 [Cancer Immune Checkpoint Assay Kit]

CAT#: IOK-05-P174
Product Type: ELISA Kit
Target: IL1B
Short Description
The kit is designed for in vitro quantitative measurement of Human IL1B in Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate).
Description
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IL-1 beta
Applications
ELISA
Application Notes
Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Comment
Sequence similarities: Belongs to the IL-1 family.
Target
IL1B
Reactivity
Human
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Analytical Method
Quantitative
Sample Type
Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate)
Specificity
Expression system for standard: E.coli,A117-S269
Cross-Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Binding Specificity
AA 117-269
Material not included
Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Sensitivity
<0.15pg/mL
Plate
Pre-coated
Assay Procedure
Aliquot 0.1 mL per well of the 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 16.5pg/mL, 7.8pg/mL, 3.9pg/mL human IL-1 betastandard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernatants, serum or plasma(heparin, EDTA, citrate) to each empty well.
Assay Precision
Sample 1: n=16, Mean(pg/ml): 19, Standard deviation: 1.0, CV(%): 5.3
Sample 2: n=16, Mean(pg/ml): 41.2, Standard deviation: 2.6, CV(%): 6.3
Sample 3: n=16, Mean(pg/ml): 116, Standard deviation: 6.5, CV(%): 5.6
Sample 1: n=24, Mean(pg/ml): 27.5, Standard deviation: 1.6, CV(%): 5.8
Sample 2: n=24, Mean(pg/ml): 61.3, Standard deviation: 4.1, CV(%): 6.7
Sample 3: n=24, Mean(pg/ml): 179.1, Standard deviation: 12.7, CV(%): 7.1
Handling Advice
Avoid multiple freeze-thaw cycles.
Storage
-20 °C,4 °C
Storage Comment
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date
12 months
Note
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Restrictions
For Research Use only
Alternative Name
Interleukin 1 Beta (IL1b)
Synonyms
IL-1; IL1-BETA; IL1F2; IL-1BETA; IL1beta; il1-b; zgc:111873; IL-1B; IL-1beta; Il-1b; IL1B; IL-1 beta; IL-1b; interleukin 1 beta; interleukin 1; beta; IL1B; il1b; Il1b
Background
Interleukin-1beta(IL-1beta) is a potent stimulator of bone resorption whose gene is mapped to 2q14, and has been implicated in the pathogenesis of high bone turnover and osteoporosis. IL-1beta, a prominent microglia-derived cytokine, caused oligodendrocyte death in coculture with astrocytes and microglia, but not in pure culture of oligodendrocytes alone.
Gene ID
3553
UniProt
P01584
Pathways
NF-kappaB Signaling, Interferon-gamma Pathway, TLR Signaling, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagy, Cancer Immune Checkpoints, Inflammasome
Protocol
A monoclonal antibody from mouse specific for IL-1 beta has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-1 beta is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution.
For Research Use Only | Not For Clinical Use
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