Short Decsription
The kit is designed for in vitro quantitative measurement of Human IL1R1 in Cell Culture Supernatant, Plasma, Serum, Tissue Lysate.
Description
For quantitative detection of IL-1RA in human serum, plasma, body fluids, tissue lysates or cell culture supernatants.
Comment
This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- IL-1RA polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- IL-1RA polyclonal antibody was used as detection antibodies. The standards test samples and biotin conjugated detection antibody were added - the wells subsequently and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used - visualize HRP enzymatic reaction. TMB was catalyzed by HRP - produce a blue color product that changed into yellow after adding acidic stop solution. Read the O.D. absorbance at 450 nm in a microplate reader and then the concentration of IL-1RA can be calculated.
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Analytical Method
Quantitative
Sample Type
Cell Culture Supernatant, Plasma, Serum, Tissue Lysate
Datasheet