Human IL6RA ELISA Kit, Lot 21AO-1343 [Cancer Immune Checkpoint Assay Kit]

CAT#: IOK-05-P1254
Product Type: ELISA Kit
Target: IL6RA
Short Description
The kit is designed for in vitro quantitative measurement of Human IL6RA in Cell Culture Supernatant, Plasma (EDTA - heparin - citrate), Serum.
Description
Sandwich ELISA for Quantitative Detection of Antigen
Applications
ELISA
Application Notes
Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human IL-6R alpha standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA, citrate) or urine to each empty well.
Blood Product Anticoagulant: Heparin Sodium.
ELISA Dilution: 31.2pg/mL-2000pg/mL.
Target
IL6RA
Reactivity
Human
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Analytical Method
Quantitative
Sample Type
Cell Culture Supernatant, Plasma (EDTA - heparin - citrate), Serum
Sample Volume
100 μL
Plate
Pre-coated
Storage
RT,4 °C,-20 °C
Storage Comment
Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
Note
Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
Restrictions
For Research Use only
Alternative Name
Interleukin-6 receptor subunit alpha
Synonyms
IL6R1; Il6ra; CD126; IL-6R-1; IL-6RA; IL6Q; IL6RA; IL6RQ; gp80; IL-6R; Il6r; interleukin 6 receptor; interleukin 6 receptor; alpha; Il6r; IL6R; Il6ra
Gene ID
3570
UniProt
A0N0L5
Pathways
JAK-STAT Signaling, Autophagy, Growth Factor Binding, Cancer Immune Checkpoints
For Research Use Only | Not For Clinical Use
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