Human IL6ST ELISA Kit, Lot 21AO-1381 [Cancer Immune Checkpoint Assay Kit]

The kit is designed for in vitro quantitative measurement of Human IL6ST in Cell Culture Supernatant, Plasma, Serum.

The kit is a sandwich enzyme immunoassay technique for the in vitro quantitative measurement in various sample types.

ELISA

IL6ST

Human

Colorimetric

Sandwich ELISA

Quantitative

Cell Culture Supernatant, Plasma, Serum

This kit recognizes Human gp130 in samples. No significant cross-reactivity or interference between Human gp130 and analogues was observed.

Pre-coated, ready to use 96-well strip plate
Plate sealer for 96 wells
Standard
Sample/Standard Dilution Buffer
Biotin-labeled Antibody (Concentrated)
Antibody Dilution Buffer
HRP-Streptavidin Conjugate (SABC)
SABC Dilution Buffer
TMB Substrate
Stop Solution
Wash Buffer (25 x concentrate)
Instruction manual

46.88 pg/mL

100 μL

3.5 h

Pre-coated

1.Bring all reagents to room temperature (18-25 °C) before use.
2.Wash Buffer: Dilute 30 mL of Concentrated Wash Buffer with 720 mL of deionized or distilled water to prepare 750 mL of Wash Buffer.
3.Standard working solution: Add 1.0 mL of Reference Standard &Sample Diluent, let it stand for 10 min and invert it gently several times. After it dissolves fully, mix it thoroughly with a pipette. This reconstitution produces a working solution of 200pg/mL.
4.Biotinylated Detection Ab working solution: Centrifuge the Concentrated Biotinylated Detection Ab at 800xg for 1 min, then dilute the 100x Concentrated Biotinylated Detection Ab to 1x working solution with Biotinylated Detection Ab Diluent.
5.HRP Conjugate working solution: Calculate the required amount before the experiment.

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human gp130 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human gp130 were tested on 3 different plates, 20 replicates in each plate.
Both intra-CV and inter-CV are < 10 %.

4 °C,-20 °C

6 months

For Research Use only

Glycoprotein 130 (gp130)

CD130; CDW130; GP130; IL-6RB; 5133400A03Rik; AA389424; BB405851; D13Ertd699e; gp130; Ac1055; Gp130; Il-6rb; IL-6; interleukin 6 signal transducer; IL6ST; Il6st; il6st

IL6ST, CD130, CDW130, IL-6RB, IR6RB, Interleukin 6 signal transducer

3572

P40189

JAK-STAT Signaling, Cellular Glucan Metabolic Process, Autophagy, Smooth Muscle Cell Migration, Cancer Immune Checkpoints

1.Add 100 μL standard or sample to each well. Incubate for 90 min at 37 °C.
2.Remove the liquid. Add 100 μL Biotinylated Detection Ab. Incubate for 1 hour at 37 °C.
3.Aspirate and wash 3 times.
4.Add 100 μL HRP Conjugate. Incubate for 30 min at 37 °C.
5.Aspirate and wash 5 times.
6.Add 90 μL Substrate Reagent. Incubate for 15 min at 37 °C.
7.Add 50 μL Stop Solution. Read at 450 nm immediately.