Human LGALS9 ELISA Kit, Lot 21AO-206 [Cancer Immune Checkpoint Assay Kit]

CAT#: IOK-05-P117
Product Type: ELISA Kit
Target: LGALS9
Short Description
The kit is designed for in vitro quantitative measurement of Human LGALS9 in Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA).
Description
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Galectin-9
Applications
ELISA
Application Notes
Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Comment
Sequence similarities: Contains 2 galectin domains.
Tissue Specificity: Peripheral blood leukocytes and lymphatic tissues. Overexpressed in Hodgkin disease tissue.
Target
LGALS9
Reactivity
Human
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Analytical Method
Quantitative
Sample Type
Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
Specificity
Expression system for standard: E.coli; Immunogen sequence: M1-T355
Cross-Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Binding Specificity
AA 1-355
Material not included
Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Sensitivity
<10pg/mL
Plate
Pre-coated
Assay Procedure
Aliquot 0.1 mL per well of the 6000pg/mL, 3000pg/mL, 1500pg/mL, 750pg/mL, 375pg/mL, 187.5pg/mL, 93.7pg/m human Galectin-9 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well.
Assay Precision
Sample 1: n=16, Mean(pg/ml): 526, Standard deviation: 23.67, CV(%): 4.5
Sample 2: n=16, Mean(pg/ml): 2335, Standard deviation: 91.07, CV(%): 3.9
Sample 3: n=16, Mean(pg/ml): 4518, Standard deviation: 189.8, CV(%): 4.2
Sample 1: n=24, Mean(pg/ml): 521, Standard deviation: 38.03, CV(%): 7.3
Sample 2: n=24, Mean(pg/ml): 2357, Standard deviation: 143.8, CV(%): 6.1
Sample 3: n=24, Mean(pg/ml): 4526, Standard deviation: 294.2, CV(%): 6.5
Handling Advice
Avoid multiple freeze-thaw cycles.
Storage
-20 °C,4 °C
Storage Comment
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date
12 months
Note
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Restrictions
For Research Use only
Alternative Name
Galectin 9 (GAL9)
Synonyms
huat; lgals9a; ecalectin; galectin-9; Lgals9; wu:fd20c09; zgc:111833; LGALS9; AA407335; AI194909; AI265545; LGALS35; Lgals5; gal-9; HUAT; LGALS9A; UAT; UATP.I; galectin 9 L homeolog; galectin 9; lectin; galactoside-binding; soluble; 9 (galectin 9)-like 3; lectin; galactose binding; soluble 9; lgals9.L; lgals9; lgals9l3; LGALS9; Lgals9
Background
Galectin-9, also called HUAT or LGALS9A is a protein that in humans is encoded by the LGALS9 gene. This gene is mapped to 17q11.2. The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. The protein encoded by this gene is an S-type lectin.
Gene ID
3965
UniProt
O00182
Protocol
A monoclonal antibody from mouse specific for Galectin-9 has been precoated onto 96-well plates. Standards(E.coli, M1-T355) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Galectin-9 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution.
For Research Use Only | Not For Clinical Use
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