Human MICA ELISA Kit, Lot 21AO-1089 [Cancer Immune Checkpoint Assay Kit]

The kit is designed for in vitro quantitative measurement of Human MICA in Cell Culture Supernatant, Plasma, Serum.

Human Immunoquantitative (PCR-Based) MICA ELISA Kit for cell culture supernatants, plasma, and serum samples.

ELISA

The Immuno-Quantitative ELISA kits are an innovative assay platform that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR. This results in an assay that is simultaneously familiar and cutting edge and enables the use of only 1/10th the sample volume while also providing 10x more sensitivity than a traditional ELISA.

The Immuno-Quantitative ELISA kits are an innovative assay platform that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR. Also called immuno-PCR, this detection platform results in an assay that is simultaneously familiar and cutting edge. Compared to traditional ELISA, quantitative ELISA enables the use of only 1/10th the sample volume while also providing 10x more sensitivity.

MICA

Human

qPCR

Sandwich ELISA

Semi-Quantitative

Cell Culture Supernatant, Plasma, Serum

MICA Microplate (Item A): 96 well PCR plate coated with anti-Human MICA.
Wash Buffer I Concentrate (20x) (Item B): 25 ml of 20x concentrated solution.
Standards (Item C): 2 vials of recombinant Human MICA.
Assay Diluent A (Item D): 30 ml diluent buffer, 0.09% sodium azide as preservative.
Assay Diluent B (Item E): 15 ml of 5x concentrated buffer.
Detection Affinity Reagent for MICA (Item F): 2 vials of a 4x concentrated solution of anti-Human MICA affinity reagent.
Quantitative ELISA Detection Reagent (Item G): 1.4ml of a 10x concentrated stock.
Primer Solution (Item I): 1.7 ml vial.
PCR Master Mix (Item J): 1.2 ml vial.
PCR Preparation buffer (Item K): 1ml vial of 10x concentrated buffer.
Final Wash Buffer (Item L): 10ml vial of 10x concentrated buffer.

Real-time PCR instrument, Bio-Rad recommended.
Precision pipettes to deliver 2 μL to 1 mL volumes.
Adjustable 1-25 mL pipettes for reagent preparation.
100 mL and 1 liter graduated cylinders.
Absorbent paper.
Distilled or deionized water.
Log-log graph paper or computer and software for data analysis.
Tubes to prepare standard or sample dilutions.
Heating block or water bath capable of 80°C.

2 pg/mL

25 µL

Pre-coated

4 °C,-20 °C,-80 °C

May be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standard (recombinant protein) should be stored at -20°C or -80°C (recommended at -80°C) after reconstitution. Opened PCR plate or reagents may be stored for up to 1 month at 2°C to 8°C.

6 months

May be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standard (recombinant protein) should be stored at -20°C or -80°C (recommended at -80°C) after reconstitution. Opened PCR plate or reagents may be stored for up to 1 month at 2°C to 8°C.

For Research Use only

MHC class I Chain-related gene A

MIC-A; PERB11.1; MHC class I polypeptide-related sequence A; MICA

100507436

Q29983

Activation of Innate immune Response, Transition Metal Ion Homeostasis, Human Leukocyte Antigen (HLA) in Adaptive Immune Response

1.Prepare all reagents, samples and standards as instructed.
2.Add 25 μL standard or sample to each well. Incubate for 2.5 hours at room temperature or overnight at 4°C.
3.Add 25 μL detection affinity reagent to each well. Incubate 1 hour at room temperature.
4.Add 25μL of quantitative ELISA Detection Reagent to each well. Incubate 1 hour.
5.Add 15μL Primer solution 10μL of PCR master mix to each well.
6.Run real-time PCR.