Human MICB ELISA Kit, Lot 21AO-1100 [Cancer Immune Checkpoint Assay Kit]

CAT#: IOK-05-P1011
Product Type: ELISA Kit
Target: MICB
Short Description
The kit is designed for in vitro quantitative measurement of Human MICB in Plasma, Serum, Tissue Homogenate.
Description
For the quantitative determination of human MHC class I polypeptide-related sequence B (MICB) concentrations in serum, plasma, tissue homogenates.
Applications
ELISA
Application Notes
Optimal working dilution should be determined by the investigator.
Target
MICB
Reactivity
Human
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Analytical Method
Quantitative
Sample Type
Plasma, Serum, Tissue Homogenate
Specificity
This assay has high sensitivity and excellent specificity for detection of human MICB. No significant cross-reactivity or interference between human MICB and analogues was observed.
Cross-Reactivity
Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between human MICB and all the analogues, therefore, cross reaction may still exist.
Components
Assay plate
Standard
HRP-avidin (100 x concentrate)
Biotin-antibody (100 x concentrate)
Sample Diluent
HRP-avidin Diluent
Biotin-antibody Diluent
Wash Buffer (25 x concentrate)
TMB Substrate
Stop Solution
Adhesive Strip
Sensitivity
7.8 pg/mL
Sample Volume
100 μL
Assay Time
1 - 4.5 h
Plate
Pre-coated
Reagent Preparation
Biotin-antibody (1×) - Centrifuge the vial before opening.
HRP-avidin (1×) - Centrifuge the vial before opening.
Wash Buffer (1×) - If crystals have formed in the concentrate, warm up to room temperature and mix gently until the crystals have completely dissolved.
Standard - Centrifuge the standard vial at 6000-10000rpm for 30s.
Calculation of Results
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess.
Storage
4 °C,-20 °C
Storage Comment
Unopened kit Store at 2 - 8°C. Do not use the kit beyond the expiration date. May be stored for up to 1 month at 2 - 8°C. Coated assay Try to keep it in a sealed aluminum foil bag, plate and avoid the damp. Standard May be stored for up to 1 month at 2 - 8°C. If Biotin-antibody doesn't make recent use, better keep it store at HRP-avidin -20°C.
Expiry Date
6 months
Note
Unopened kit Store at 2 - 8°C. Do not use the kit beyond the expiration date. May be stored for up to 1 month at 2 - 8°C. Coated assay Try to keep it in a sealed aluminum foil bag, plate and avoid the damp. Standard May be stored for up to 1 month at 2 - 8° C. If Biotin-antibody don't make recent use, better keep it store at HRP-avidin -20°C.
Restrictions
For Research Use only
Alternative Name
MHC Class I Polypeptide-Related Sequence B
Synonyms
PERB11.2; MHC class I polypeptide-related sequence B; MHC class I-related protein-like; MICB; LOC512825
Background
Abbreviation: MICB
Alias: DAAP-210H10.1, PERB11.2, MHC class I chain-related protein B|MHC class I mic-B antigen|MHC class I molecule|MHC class I-like molecule PERB11.2-IMX|stress inducible class I homolog
Gene ID
4277
UniProt
Q29980
Pathways
Human Leukocyte Antigen (HLA) in Adaptive Immune Response
For Research Use Only | Not For Clinical Use
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