Human NF-kappa B-Luciferase Reporter Recombinant Cell Line, Jurkat (CAT#: IOC-LX147)

Creative Biolabs has developed several immune checkpoint stable cell Lines expressing the most popular immune checkpoints to accelerate clients' immunotherapy discovery and clinical translation. NF-κB luciferase reporter construct is stably integrated into the genome of Jurkat T- cells. The firefly luciferase gene is controlled by 4 copies of NF-κB response element located upstream of the TATA promoter. Following activation by stimulants, endogenous NF-κB transcription factors bind to the DNA response elements to induce transcription of the luciferase gene. Which can be used in binding assays and functional assay.

Stable cell lines for increased reliability and reproducibility;
Protein expression at the cell surface validated by FACS or WB;

The NF-kappaB-luciferase/Jurkat cell line is suitable for monitoring the activity of NF-kB signaling in response to stimulant, and establishing cell-based screens for inhibitors that target specific NF-kappaB stimulating molecules.

Human

Jurkat

NF-kappa B-Luciferase Reporter

The nuclear factor of activator T cells (NFAT) family of transcription factors plays an important role in immune response. T cell activation through the T cell synapse results in calcium influx. Increased intracellular calcium levels activate the calcium-sensitive phosphatase, calcineurin, which rapidly dephosphorylates the serine-rich region (SRR) and SP-repeats in the amino termini of NFAT proteins. This results in a conformational change that exposes a nuclear localization signal promoting NFAT nuclear import. In the nucleus, NFAT proteins cooperate with other proteins to bind to DNA.