Human PD-1 Stable Cell Line, Daudi

CAT#: IOC-LX043
Product Type: Cell
Organism: Human
Description
Creative Biolabs has developed several immune checkpoint stable cell Lines expressing the most popular immune checkpoints to accelerate clients' immunotherapy discovery and clinical translation. Human PD-1 Stable Cell Line, Daudi is constructed to constitutively expressing Human PD-1. Which can be used in binding assays and functional assay.
Features
Stable cell lines for increased reliability and reproducibility;
Protein expression at the cell surface validated by FACS or WB;
Applications
The cell line can be used in binding assay or function assay in vitro.
Organism
Human
Strain Name
Daudi
Common Name
PD-1
Background
Programmed cell death protein 1, also known as PD-1 and CD279 (cluster of differentiation 279), is a protein that in humans is encoded by the PDCD1 gene. PD-1, functioning as an immune checkpoint, plays an important role in down regulating the immune system by preventing the activation of T-cells, which in turn reduces autoimmunity and promotes self-tolerance. The inhibitory effect of PD-1 is accomplished through a dual mechanism of promoting apoptosis (programmed cell death) in antigen specific T-cells in lymph nodes while simultaneously reducing apoptosis in regulatory T cells (suppressor T cells).
Shipping Condition
Gel Packs
Unit Size
Containing ≥ 1 X 10*6 / vial lyophilized cells
Culture Medium
RPMI Medium 1640, 10% FBS, 0.5 μg/ml Puromycin
Frozen Formulation
95% complete growth medium, 5% DMSO
Cell Purity
>95%
Cell Viability
>90%
Sterility Testing
Creative Biolabs provides sterility testing in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.
Identity Testing
Identity testing is required for newly established cell lines. Isoenzyme analysis is used to confirm the identity of the species of a cell line. Alternative methods for identity testing include DNA fingerprinting, STR analysis and karyology.
Virological Safety Testing
A broad range of viruses is susceptible to affecting human cell lines. We can provide in vivo/vitro virus safety assays by utilizing various animal systems. These viruses include: adventitious viruses, bovine viruses, human and simian viruses, porcine viruses, retrovirus and rodent viruses.
Genetic Stability Testing
We perform cell genetic stability studies under ICH guidelines. We can provide guidance on the appropriate testing program upon your requirements.
Mycoplasma Testing
The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.
Shipping
Dry Ice
Storage
Immediately upon receipt, store in liquid nitrogen.
Handling Notes
Frozen cells should be thawed immediately upon receipt and grown according to handling procedure to ensure cell viability and proper assay performance.
Note: Do not freeze the cells upon receipt as it may result in irreversible damage to the cell line.
Disclaimer: We cannot guarantee cell viability if the cells are not thawed immediately upon receipt and grown according to handling procedure.
Warnings
Avoid multiple freeze/thaw cycles
Research Use Only
For research use only, not for diagnostic or therapeutic use.
Quality Control
All of our bioluminescent/reporter tumor cell lines meet the highest quality standards and have been confirmed to be pathogen-free.
Passage Stability
15 passages
For Research Use Only | Not For Clinical Use
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