Human, Rhesus Monkey IL6R ELISA Kit, Lot 21AO-1360 [Cancer Immune Checkpoint Assay Kit]

CAT#: IOK-05-P1271
Product Type: ELISA Kit
Target: IL-6 Receptor
Short Description
The kit is designed for in vitro quantitative measurement of Human, Rhesus Monkey IL6R in Plasma, Cell Culture Supernatant, Serum.
Description
Rhesus Monkey IL-6 R ELISA Kit for cell culture supernatants, plasma, and serum samples.
Applications
ELISA
Application Notes
Recommended Dilution for serum and plasma samples: 20 - 200-fold
Target
IL-6 Receptor
Reactivity
Human, Rhesus Monkey
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Analytical Method
Quantitative
Sample Type
Plasma, Cell Culture Supernatant, Serum
Specificity
This ELISA antibody pair detects Rhesus Monkey and human IL-6R. Other species not determined.
Components
Pre-Coated 96-well Strip Microplate
Wash Buffer
Stop Solution
Assay Diluent(s)
Lyophilized Standard
Biotinylated Detection Antibody
Streptavidin-Conjugated HRP
TMB One-Step Substrate
Material not included
Distilled or deionized water
Precision pipettes to deliver 2 μL to 1 μL volumes
Adjustable 1-25 μL pipettes for reagent preparation
100 μL and 1 liter graduated cylinders
Tubes to prepare standard and sample dilutions
Absorbent paper
Microplate reader capable of measuring absorbance at 450nm
Log-log graph paper or computer and software for ELISA data analysis
Sensitivity
12 pg/mL
Sample Volume
100 μL
Plate
Pre-coated
Assay Procedure
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Storage
-20 °C
Storage Comment
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date
6 months
Note
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Restrictions
For Research Use only
Alternative Name
IL-6 Receptor (IL6R)
Synonyms
IL6R; il-6ra; CD126; IL-6R-1; IL-6RA; IL6Q; IL6RA; IL6RQ; gp80; IL6R1; Il6ra; IL-6Ralpha; IL-6R; Il6r; interleukin 6 receptor; interleukin 6 receptor; alpha; IL6R; il6r; Il6r; Il6ra
Background
Interleukin-6 receptor subunit alpha isoform 1 (Fragment)
UniProt
H9FAX3
Pathways
JAK-STAT Signaling, Autophagy, Growth Factor Binding, Cancer Immune Checkpoints
Protocol
1.Prepare all reagents, samples and standards as instructed in the manual.
2.Add 100 μL of standard or sample to each well.
3.Incubate 2.5 h at RT or O/N at 4°C.
4.Add 100 μL of prepared biotin antibody to each well.
5.Incubate 1 h at RT.
6.Add 100 μL of prepared Streptavidin solution to each well.
7.Incubate 45 min at RT.
8.Add 100 μL of TMB One-Step Substrate Reagent to each well.
9.Incubate 30 min at RT.
10.Add 50 μL of Stop Solution to each well.
11.Read at 450 nm immediately.
For Research Use Only | Not For Clinical Use
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