Human T Cell Receptor ELISA Kit, Lot 21AO-985 [Cancer Immune Checkpoint Assay Kit]

The kit is designed for in vitro quantitative measurement of Human T Cell Receptor in Cell Culture Supernatant, Tissue Samples.

ELISA

Further details regarding sample type: Plasma, Cell and tissue culture supernatants, Serum, Other biological fluids

T Cell Receptor

Human

Colorimetric

Sandwich ELISA

Quantitative

Cell Culture Supernatant, Tissue Samples

Standard (4000 pg/ml), Stop Solution, Sample diluent, Standard diluent, Micro ELISA strip-plate, Chromogen Solution B, Plate sealers, HRP-Conjugate reagent, Chromogen Solution A, Wash solution (20X)

10 pg/mL

Intra-Assay: CV<10 % , Inter-Assay: CV<12 %, CV (%) = SD/mean X 100

4 °C

12 months

For Research Use only

T cell receptor

Gm13948; Gm193; Gm4; OTTMUSG00000015160; TCR; T cell receptor alpha variable 6-3; Trav6-3

The Micro Elisa strip plate provided in this kit has been pre-coated with an antibody specific to TCR. Standards or samples are added to the appropriate Micro Elisa strip plate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP) conjugated antibody specific for TCR is added to each Micro Elisa strip plate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. The wells that contain TCR and HRP conjugated TCR antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm.