Human TIGIT ELISA Kit, Lot 21AO-1171 [Cancer Immune Checkpoint Assay Kit]

CAT#: IOK-05-P1082
Product Type: ELISA Kit
Target: TIGIT
Short Description
The kit is designed for in vitro quantitative measurement of Human TIGIT in Plasma, Cell Culture Supernatant, Serum.
Description
Human TIGIT ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.
Applications
ELISA
Application Notes
Recommended Dilution for serum and plasma samples: 2-fold
Target
TIGIT
Reactivity
Human
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Analytical Method
Quantitative
Sample Type
Plasma, Cell Culture Supernatant, Serum
Specificity
This ELISA antibody pair recognizes Human TIGIT.
Cross-Reactivity
Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between human B7-H4 and all the analogues, therefore, cross reaction may still exist.
Components
Pre-Coated 96-well Strip Microplate
Wash Buffer
Stop Solution
Assay Diluent(s)
Lyophilized Standard
Biotinylated Detection Antibody
Streptavidin-Conjugated HRP
TMB One-Step Substrate
Material not included
Distilled or deionized water
Precision pipettes to deliver 2 μL to 1 μL volumes
Adjustable 1-25 μL pipettes for reagent preparation
100 μL and 1 liter graduated cylinders
Tubes to prepare standard and sample dilutions
Absorbent paper
Microplate reader capable of measuring absorbance at 450nm
Log-log graph paper or computer and software for ELISA data analysis
Sensitivity
1.5 ng/mL
Sample Volume
100 μL
Plate
Pre-coated
Storage
-20 °C
Storage Comment
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date
6 months
Note
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Restrictions
For Research Use only
Alternative Name
T-Cell Immunoreceptor With Ig And ITIM Domains Protein (TIGIT)
Synonyms
VSIG9; VSTM3; WUCAM; ENSMUSG00000071552; Vstm3; RGD1563191; T-cell immunoreceptor with Ig and ITIM domains; T cell immunoreceptor with Ig and ITIM domains; TIGIT; Tigit
Gene ID
201633
UniProt
Q495A1
Pathways
Cancer Immune Checkpoints
Protocol
1.Prepare all reagents, samples and standards as instructed in the manual.
2.Add 100 μL of standard or sample to each well.
3.Incubate 2.5 h at RT or O/N at 4°C.
4.Add 100 μL of prepared biotin antibody to each well.
5.Incubate 1 h at RT.
6.Add 100 μL of prepared Streptavidin solution to each well.
7.Incubate 45 min at RT.
8.Add 100 μL of TMB One-Step Substrate Reagent to each well.
9.Incubate 30 min at RT.
10.Add 50 μL of Stop Solution to each well.
11.Read at 450 nm immediately.
For Research Use Only | Not For Clinical Use
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