Human TLR9/NF-κB Reporter Recombinant Cell Line, HEK293 (CAT#: IOC-LX141)

Creative Biolabs has developed several immune checkpoint stable cell Lines expressing the most popular immune checkpoints to accelerate clients' immunotherapy discovery and clinical translation. Recombinant HEK293 cell expressing firefly luciferase gene under the control of NF-κB response elements with constitutive expression of human TLR9 (Toll-like receptor 9, also been designated as CD289, a member of the toll-like receptor (TLR) family. GenBank Accession No. NM_017442). Which can be used in binding assays and functional assay.

Stable cell lines for increased reliability and reproducibility;
Protein expression at the cell surface validated by FACS or WB;

Screen for activators or inhibitors of TLR9 signaling in a cellular context and characterize the biological activity of TLR9 and its interactions with ligands.

Human

HEK293

TLR9/NF-κB Reporter

The family of Toll-like receptors (TLRs) acts as primary sensors that detect a wide variety of microbial components and elicit innate immune responses.
Human TLR9 recognizes specific unmethylated CpG DNA motifs prevalent in microbial but not vertebrate genomic DNA, leading to innate and acquired immune responses. Stimulation of TLR9 triggers a signaling cascade leading to the activation of the transcription factor NF-κB, which controls the expression of an array of inflammatory cytokine genes.