Human TNFRSF18 ELISA Kit, Lot 21AO-733 [Cancer Immune Checkpoint Assay Kit]

CAT#: IOK-05-P644
Product Type: ELISA Kit
Target: TNFRSF18
Short Description
The kit is designed for in vitro quantitative measurement of Human TNFRSF18 in Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA).
Description
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human TNFRSF18/GITR
Applications
ELISA
Application Notes
Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Comment
Tissue Specificity: Expressed in lymph node, peripheral blood leukocytes and weakly in spleen.
Target
TNFRSF18
Reactivity
Human
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Analytical Method
Quantitative
Sample Type
Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
Specificity
Expression system for standard: NSO; Immunogen sequence: Q26-E161
Cross-Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Binding Specificity
AA 26-161
Material not included
Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Sensitivity
<10pg/mL
Plate
Pre-coated
Assay Procedure
Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human TNFRSF18 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well.
Assay Precision
Sample 1: n=16, Mean(pg/ml): 342, Standard deviation: 11.97, CV(%): 3.5.
Sample 2: n=16, Mean(pg/ml): 1565, Standard deviation: 62.6, CV(%): 4.
Sample 3: n=16, Mean(pg/ml): 3014, Standard deviation: 153.7, CV(%): 5.1.
Sample 1: n=24, Mean(pg/ml): 540, Standard deviation: 30.24, CV(%): 5.6.
Sample 2: n=24, Mean(pg/ml): 1896, Standard deviation: 110, CV(%): 5.8.
Sample 3: n=24, Mean(pg/ml): 3242, Standard deviation: 217.2, CV(%): 6.7.
Handling Advice
Avoid multiple freeze-thaw cycles.
Storage
-20 °C,4 °C
Storage Comment
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date
12 months
Note
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Restrictions
For Research Use only
Alternative Name
Glucocorticoid Induced Tumor Necrosis Factor Receptor (GITR)
Synonyms
GITR; TNFRSF18; AITR; Gitr; CD357; GITR-D; TNF receptor superfamily member 18; tumor necrosis factor receptor superfamily; member 18; TNFRSF18; Tnfrsf18
Gene ID
8784
UniProt
Q9Y5U5
Pathways
Cancer Immune Checkpoints
Protocol
A monoclonal antibody from mouse specific for TNFRSF18 has been precoated onto 96-well plates. Standards(NSO, Q26-E161) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNFRSF18 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution.
For Research Use Only | Not For Clinical Use
Online Inquiry