Human VEGF ELISA Kit, Lot 21AO-937 [Cancer Immune Checkpoint Assay Kit]

The kit is designed for in vitro quantitative measurement of Human VEGF in Cell Culture Supernatant, Plasma, Serum.

Human Immunoquantitative (PCR-Based) VEGF ELISA Kit for cell culture supernatants, plasma, and serum samples.

ELISA

The Immuno-Quantitative ELISA kits are an innovative assay platform that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR. This results in an assay that is simultaneously familiar and cutting edge and enables the use of only 1/10th the sample volume while also providing 10x more sensitivity than a traditional ELISA.

Detection of quantitative protein levels in biological fluids.
Validation of antibody array results.
Validation of biomarker discovery studies.

VEGF

Human

qPCR

Sandwich ELISA

Semi-Quantitative

Cell Culture Supernatant, Plasma, Serum

VEGF Microplate (Item A): 96 well PCR plate coated with anti-human VEGF.
Wash Buffer I Concentrate (20x) (Item B): 20x concentrated solution.
Standards (Item C): recombinant human VEGF.
Detection Affinity Reagent for VEGF (Item F): 4x concentrated solution of anti-human VEGF affinity reagent.
Primer Solution (Item G).
PCR Master Mix (Item H).
PCR Preparation buffer (Item I): 10x concentrated buffer.
Final Wash Buffer (Item J): 10x concentrated buffer.
Assay Diluent A (Item D): diluent buffer, 0.09% sodium azide as preservative.
Assay Diluent B (Item E): 5x concentrated buffer.

Real-time PCR instrument, Bio-Rad recommended.
Precision pipettes to deliver 2 μL to 1 mL volumes.
Adjustable 1-25 mL pipettes for reagent preparation.
100 mL and 1 liter graduated cylinders.
Absorbent paper.
Distilled or deionized water.
Log-log graph paper or computer and software for data analysis.
Tubes to prepare standard or sample dilutions.
Heating block or water bath capable of 80°C.

1 pg/mL

Pre-coated

Serum spike tests show recovery is 93.3% with a range of 89.7 to 98.0%.

4 °C,-20 °C,-80 °C

May be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standard (recombinant protein) should be stored at -20°C or -80°C (recommended at -80°C) after reconstitution. Opened PCR plate or reagents may be stored for up to 1 month at 2°C to 8

6 months

May be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standard (recombinant protein) should be stored at -20°C or -80°C (recommended at -80°C) after reconstitution. Opened PCR plate or reagents may be stored for up to 1 month at 2° to 8

For Research Use only

Vascular Endothelial Cell Growth Factor

MVCD1; VEGF; VPF; Vegf; Vegf120; Vegf164; Vegf188; Vpf; VEGF-A; VEGF164; eVEGF120; eVEGF164; vegf-a; vegf; vascular endothelial growth factor A; vascular endothelial growth factor A L homeolog; vascular endothelial growth factor; vascular endothelial growth factor precursor; VEGFA; Vegfa; vegfa.L; VEGF; vegf

Synonyms: Vascular endothelial growth factor A (VEGF-A) (Vascular permeability factor) (VPF), VEGFA VEGF

7422

P15692

RTK Signaling, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling

1.Prepare all reagents, samples and standards as instructed.
2.Add 25 μL standard or sample to each well. Incubate for 2.5 hours at room temperature or overnight at 4°C.
3.Add 25 μL detection affinity reagent to each well. Incubate 1 hour at room temperature.
4.Add 25μL of quantitative ELISA Detection Reagent to each well. Incubate 1 hour.
5.Add 15μL Primer solution 10μL of PCR master mix to each well.
6.Run real-time PCR.