Components
VEGF Microplate (Item A): 96 well PCR plate coated with anti-human VEGF.
Wash Buffer I Concentrate (20x) (Item B): 20x concentrated solution.
Standards (Item C): recombinant human VEGF.
Detection Affinity Reagent for VEGF (Item F): 4x concentrated solution of anti-human VEGF affinity reagent.
Primer Solution (Item G).
PCR Master Mix (Item H).
PCR Preparation buffer (Item I): 10x concentrated buffer.
Final Wash Buffer (Item J): 10x concentrated buffer.
Assay Diluent A (Item D): diluent buffer, 0.09% sodium azide as preservative.
Assay Diluent B (Item E): 5x concentrated buffer.
Material not included
Real-time PCR instrument, Bio-Rad recommended.
Precision pipettes to deliver 2 μL to 1 mL volumes.
Adjustable 1-25 mL pipettes for reagent preparation.
100 mL and 1 liter graduated cylinders.
Absorbent paper.
Distilled or deionized water.
Log-log graph paper or computer and software for data analysis.
Tubes to prepare standard or sample dilutions.
Heating block or water bath capable of 80°C.
Assay Procedure
Serum spike tests show recovery is 93.3% with a range of 89.7 to 98.0%
Storage
4 °C,-20 °C,-80 °C
Storage Comment
May be stored for up to 6 months at 2° to 8°C from the date of shipment. Standard (recombinant protein) should be stored at -20°C or -80°C (recommended at -80°C) after reconstitution. Opened PCR plate or reagents may be stored for up to 1 month at 2° to 8
Note
May be stored for up to 6 months at 2° to 8°C from the date of shipment. Standard (recombinant protein) should be stored at -20°C or -80°C (recommended at -80°C) after reconstitution. Opened PCR plate or reagents may be stored for up to 1 month at 2° to 8
Restrictions
For Research Use only