HVEM (Human) Activator/Inhibitor Screening Assay, Jurkat (HVEM & NF-κB)-based

This assay is used to screen for agonists or antagonists of LIGHT-HVEM signaling in a physiological relevant cellular context. The assay will be customized according to the specific requirements. Please contact our scientists to discuss more details.

Customized to client's specific requirements

Jurkat

Human

Creative Biolabs has developed several immune checkpoint stable cell Lines expressing the most popular immune checkpoints to accelerate clients' immunotherapy discovery and clinical translation. The HVEM cell line is a recombinant clonal stable Jurkat T cell line expressing firefly luciferase gene under the control of 4 copies of NF-κB response elements with constitutive expression of human HVEM (Herpes Virus Entry Mediator, Tumor Necrosis Factor Receptor Superfamily Member 14, TNFRSF14, CD270, GenBank Accession No. NM_003820). Following activation by human HVEM ligand LIGHT, NF-κB transcription factors bind to the DNA response elements to induce transcription of the luciferase reporter gene. Which can be used in binding assays and functional assay.

Jurkat (HVEM & NF-κB)

Cancer

Oncology

In Vitro Activator/Inhibitor Screening Assay

Screen for agonists or antagonists of LIGHT-HVEM signaling in a physiological relevant cellular context.

Creative Biolabs has developed several I-O function assay based on our stable cell Lines expressed the most popular immune checkpoints. HVEM (Human) Activator/Inhibitor Screening Assay, Jurkat (HVEM & NF-κB)-based is applied to screen for agonists or antagonists of LIGHT-HVEM signaling in a physiological relevant cellular context. The assay will be customized according to the client's specific requirements. Please contact our scientists to discuss more details.

In Vitro Activator/Inhibitor Screening Assay; Activator/Inhibitor Screening Assay

NF-κB Reporter

Activator/Inhibitor Screening Assay

Creative Biolabs has developed several immune checkpoint stable cell Lines expressing the most popular immune checkpoints to accelerate clients' immunotherapy discovery and clinical translation. The HVEM cell line is a recombinant clonal stable Jurkat T cell line expressing firefly luciferase gene under the control of 4 copies of NF-κB response elements with constitutive expression of human HVEM (Herpes Virus Entry Mediator, Tumor Necrosis Factor Receptor Superfamily Member 14, TNFRSF14, CD270, GenBank Accession No. NM_003820). Following activation by human HVEM ligand LIGHT, NF-κB transcription factors bind to the DNA response elements to induce transcription of the luciferase reporter gene. Which can be used in binding assays and functional assay.