16,16-Dimethyl-prostaglandin E2 (16,16-dimethyl PGE2) is an antisecretory, antiulcer PGE2 analog that is resistant to metabolism. It is an active vertebrate Hematopoietic stem cells (HSCs) homeostasis critical regulator and induces an increase in HSC numbers. 16,16-dimethyl PGE2 can act through EP2/EP4 and has an interaction with the Wnt pathway.