Description
I-O™ ALDH Activity Assay Kit (Flow Cytometry) is fast and reliable method to quantify the aldehyde dehydrogenase (ALDH) activity. ALDH is a superfamily of phase-I oxidases, which are mainly responsible for the maintenance of cellular homeostasis. It has been reported that ALDH is expressed in stem/progenitor cells, including cancer-initiating cells (CIC) of various organs. This kit provide a quantitative method to detect ALDH enzyme activity in a variety of samples. In this assay, acetaldehyde is oxidized by ALDH generating NADH which then reduces a colorless probe to a colored product with strong absorbance at 450 nm.
Comment
ALDH assay protocol summary:
1. Add samples and standards to wells;
2. add reaction mix and incubate for 20-60 min;
3. analyze with a microplate reader at 5 min and 20-60 min.
Target
Aldehyde dehydrogenase (ALDH)
Detection Method
Colorimetric
Method Type
Enzyme activity
Analytical Method
Quantitative
Sample Type
Cell culture supernatant, Cell Lysate, Plasma, Serum, Tissue Culture Media
Specificity
This assay has high sensitivity and excellent specificity for detection of ALDH.
Datasheet