I-O™ Apoptosis Detection Kit, Annexin V (PE) (CAT#: ITS-0622-YT345)

I-O™ Apoptosis Detection Kit consists of Annexin V, Annexin V buffer and 7-AAD. This kit provides a quick and easy method for detecting apoptotic cells by flow cytometry. The kit is used for the combined detection of early-stage cell apoptosis (using Annexin V-PE) and late-stage cell apoptosis or necrosis (using both Annexin V-PE and 7-AAD). Annexin V has a high affinity for phosphatidylserine (PS) that is present in the inner leaflet of the plasma membrane. 7-AAD is excluded from live cells with intact membranes but penetrates dead or damaged cells and binds to double-stranded DNA with high affinity by intercalating between GC base pairs.

Flow Cytometry

Fluorescent

Cell apoptosis

Annexin V-APC Ex/Em: 650/660 nm; 7-AAD Ex/Em: 503 nm/675 nm (free form); 546 nm/647 nm (DNA-complex).

Drug Discovery and Toxicity Testing, Biology, Immunology

Cells, tissue