Collagenase is a protease that cleaves the bond between a neutral amino acid (X) and glycine in the sequence Pro-X-Glyc-Pro, which is found with high frequency in collagen. The collagenase type I (from Clostridium histolyticum) is a crude collagenase preparation that can be used for the isolation of primary cells or for tissue dissociation by enzymatic means. It is recommended for cell preparation from epithelial, liver, lung tissue, tissue of the suprarenal gland and adipose tissue.
Applications
Mammalian Cell Culture; Release of cells from animal tissues