ELISA Kit for quantitative detection of human IL-17a
Applications
ELISA
Qualified With
IL-17A
Reactivity
Human
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Analytical Method
Quantitative
Sample Type
Biological fluids such as serum, plasma, and cell culture supernatants.
Specificity
This assay has high sensitivity and excellent specificity for detection of IL-17A. No significant cross-reactivity or interference between IL-17A and analogues was observed. Note: Limited by current skills and knowledge, it is difficult for us to complete the cross-reactivity detection between IL-17A and all the analogues, therefore, cross reaction may still exist.
Components
Pre-coated, ready to use 96-well strip plate Plate sealer for 96 wells Standard Sample/Standard Dilution Buffer Biotin-labeled Antibody (Concentrated) Antibody Dilution Buffer HRP-Streptavidin Conjugate (SABC) SABC Dilution Buffer TMB Substrate Stop Solution Wash Buffer (25 x concentrate) Instruction manual
Material not included
1.Microplate reader (wavelength:450nm) 2.37 °C incubator 3.Automated plate washer 4.Precision single and multi-channel pipette and disposable tips 5.Clean tubes 6.Deionized or distilled water
Sensitivity
1 pg/mL
Sample Volume
100 μL
Plate
Pre-coated
Reagent Preparation
Bring all reagents and samples to room temperature for 20 minutes before use. Wash Buffer Standards Preparation of Biotin-labeled Antibody Working Solution Preparation of HRP-Streptavidin Conjugate (SABC) Working Solution
Assay Procedure
Set standard, test samples (diluted at least 1/2 with Sample Dilution Buffer), control (blank) wells on the pre-coated plate respectively, and then, records their positions.Wash plate 2 times before adding standard, sample and control (blank) wells! Prepare Standards Add Samples Incubate Wash Biotin-labeled Antibody Wash HRP-Streptavidin Conjugate (SABC) Wash TMB Substrate Stop OD Measurement
Assay Precision
Intra-Assay: CV<3.9% Inter-Assay: CV<7.3%
Storage
Store Standard at -20°C; Store other components at 2-8°C.
Expiry Date
Stable as supplied until expiry date on label.
Note
Tested for sterility
Restrictions
Restrictions for research use only. Intended for use by laboratory professionals.
Protocol
1.Wash plate 2 times before adding Standard, Sample and Control wells! 2.Add 100 µL standard or sample to each well and incubate for 90 minutes at 37 °C. 3.Aspirate and wash plates 2 times. 4.Add 100 µL Biotin-labeled antibody working solution to each well and incubate for 60 minutes at 37 °C. 5.Aspirate and wash plates 3 times. 6.Add 100 µL SABC Working Solution into each well and incubate for 30 minutes at 37 °C. 7.Aspirate and wash plates 5 times. 8.Add 90 µL TMB Substrate Solution. Incubate 10-20 minutes at 37 °C. 9.Add 50 µL Stop Solution. Read at 450nm immediately and calculation.
