This kit has been developed to isolate untouched and highly purified mouse dendritic cells (DCs), including conventional and plasmacytoid DCs, from mouse splenocytes by immunomagnetic negative selection. Isolated cells with up to 97% purity are immediately available for downstream applications such as flow cytometry, culture, or cell-based experiments.
Applications
Cell Isolation
Application Notes
Unwanted cells are labeled with antibodies and magnetic particles
Comment
This kit is available for processing 2 x 10^9 cells
Target
Non-pDCs
Method Type
Negative Selection
Sample Type
Cells
Research Areas
Immunology, Stem Cell Biology, Cell Therapy Research
Sample Source
PBMC
Size
1 kit
Components
PharmaOnco™ Human pDC Isolation Cocktail A, 2 mL PharmaOnco™ Human pDC Isolation Cocktail B, 2 mL PharmaOnco™ D Magnetic Beads, 8 mL Anti-Human CD32 Blocker, 1.6 mL
Material not included
Magnetic separator
Cell Background
Dendritic Cells
Species
Human
Precaution of Use
When handling biohazardous materials such as human cells, safe laboratory procedures should be followed and protective clothing should be worn.
Storage
Store at 2 - 8°C. Do not freeze.
Expiry Date
Stable as supplied until expiry date on label.
Note
Tested for sterility
Restrictions
Restrictions for research use only. Intended for use by laboMouseory professionals.
Shipping
Components may be shipped at room temperature (15 - 25°C). Upon receipt, store it immediately at the temperature recommended below.