Target: CCR6+ cells, non-CD4+ T cells, CD45RA+ cells, and CXCR3+ cells
Description
This kit has been developed to enrich for human Th17 cells from fresh peripheral blood mononuclear cells. Isolated cells with up to 98% purity are immediately available for downstream applications such as flow cytometry, culture, or cryopreservation.
Applications
Cell Isolation
Application Notes
Desired cells are labeled with antibodies and magnetic particles; Unwanted cells are labeled with antibodies and magnetic particles
Comment
This kit is available for processing 2 x 10^9 cells
Target
CCR6+ cells, non-CD4+ T cells, CD45RA+ cells, and CXCR3+ cells
Method Type
Positive Selection, Negative Selection
Sample Type
Cells
Research Areas
Immunology, Stem Cell Biology, Cell Therapy Research
Sample Source
Leukapheresis, PBMC
Size
1 kit
Components
PharmaOnco™ Human CCR6 Positive Selection Cocktail II, 0.5 mL PharmaOnco™ Dextran Magnetic Beads, 1 mL PharmaOnco™ Human CD4+CXCR3- T Cell Pre-Enrichment Cocktail, 1 mL PharmaOnco™ Releasable Magnetic Beads, 4 mL PharmaOnco™ Release Buffer, 1 mL
Material not included
Magnetic separator
Cell Background
T cells, CD4+
Species
Human
Precaution of Use
When handling biohazardous materials such as human cells, safe laboratory procedures should be followed and protective clothing should be worn.
Storage
Store at 2 - 8°C. Do not freeze.
Expiry Date
Stable as supplied until expiry date on label.
Note
Tested for sterility
Restrictions
Restrictions for research use only. Intended for use by laboratory professionals.
Shipping
Components may be shipped at room temperature (15 - 25°C). Upon receipt, store it immediately at the temperature recommended below.