Product Overview
JC-1 is a novel cationic carbocyanine dye that accumulates in mitochondria. As a mitochondrial dye, JC1 stains mitochondria in living cells in a membrane potential-dependent fashion. JC-1 iodide is membrane permeable and is often used for flow cytometry and fluorescent microscopy. Green fluorescent JC-1 existing as a monomer (λem=527nm) at low concentrations or at low membrane potential. However, at higher concentrations (aqueous solutions above 0.1µM) or higher potentials, JC-1 forms red fluorescent 'J-aggregates'.
Applications
Cell Characterization
Chemical Name
5,6-Dichloro-2-[(E)-3-(5,6-dichloro-1,3-diethyl-1,3-dihydro-2H-benzimidazol-2-ylidene)-1-prop-1-enyl]-1,3-diethyl1H-benzimidazolium iodide; 5,5',6,6'-Tetrachloro-1,1',3,3'-tetraethylbenzimidazolylcarbocyanine, iodide.
Recommended Use
Fluorescence microscopy, Live cell imaging, Flow cytometry
Limitation
Restrictions for research use only. Intended for use by laboratory professionals.
Datasheet