PharmaOnco™ Megakaryocyte Differentiation Kit

PharmaOnco™ Megakaryocyte Differentiation Kit is designed for differentiation of human embryonic stem (ES) and induced pluripotent stem (iPS) cells to polyploid megakaryocytes, which express CD41a and CD41b and are capable of shedding platelets.
Use a two-step protocol to differentiate megakaryocytes ( CD41a+CD42b+) with the average frequency between 56 - 77%. During the first 12 days, PharmaOnco™ Hematopoietic Supplement A (3 days) and then Supplement MK1 (9 days) are added to basal medium to induce cells toward megakaryocyte-biased hematopoietic progenitor cells. Hematopoietic progenitor cells are harvested from the culture supernatant, and are further differentiated to mature megakaryocytes using Supplement MK2 and Hematopoietic Cells Medium II (SF) for an additional 5 days.

Serum-Free (SF) formulation

Cell Culture, Differentiation, Expansion

The products in this kit are also available for individual sale. Please contact us for your requirements.

Flow Cytometry

Cells

CD41a+CD42b+

Immunology, Stem Cell Biology, Cell Therapy Research

1 kit

PharmaOnco™ Hematopoietic Basal Medium, 120 mL
PharmaOnco™ Hematopoietic Supplement A (200X), 225 μL
PharmaOnco™ Megakaryocyte Supplement MK 1 (10X), 10 mL
PharmaOnco™ Megakaryocyte Supplement MK 2 (10X), 10 mL
PharmaOnco™ Hematopoietic Cells Medium II (SF), 100 mL

Use sterile technique to prepare Medium A (Basal Medium + Supplement A), Medium MK1 (Basal Medium + Supplement MK1), and
Medium MK2 ( Hematopoietic Cells Medium II (SF) + Supplement MK2).

Megakaryocyte

CD41a+CD42b+

Liquid

When handling biohazardous materials such as human cells, safe laboratory procedures should be followed and protective clothing should be worn.

Store at 2 - 8°C for up to 4 weeks
or Store at -20°C for up to 6 months.

Stable as supplied until expiry date on label.

Tested for sterility

Restrictions for research use only. Intended for use by laboratory professionals.