PharmaOnco™ Methylcellulose Enriched Medium

Creative Biolabs has developed a series of mediums and supplements for cell culture, expansion and differentiation. This medium is a complete methylcellulose-based medium for the growth and enumeration of hematopoietic progenitor cells in colony-forming unit (CFU) assays of human bone marrow, mobilized peripheral blood, peripheral blood, and cord blood samples. Creative Biolabs has developed a series of mediums and supplements for cell culture, expansion and differentiation. This medium supports optimal growth of erythroid progenitor cells (BFU-E and CFU-E), granulocyte-macrophage progenitor cells (CFU-GM, CFU-G and CFU-M), and multipotential granulocyte, erythroid, macrophage and megakaryocyte progenitor cells (CFU-GEMM). This product is recommended for use in the CFU assay at the end of the long-term culture-initiating cell (LTC-IC) assay.

Cell Culture, Colony Assay, Functional Assay

100 mL

Hematopoietic Stem and Progenitor Cells

Human, Non-Human Primate

Semi-Solid Media, Specialized Media

Stem Cell Biology

Restrictions for research use only. Intended for use by laboratory professionals.

Methylcellulose-based medium

Sterile-filtered

Stable until expiry date on label. Do not use past expiration date.

Store at -20°C

Methylcellulose in Iscove's MDM
Fetal bovine serum
Bovine serum albumin
2-Mercaptoethanol
Recombinant human stem cell factor (SCF)
Recombinant human interleukin 3 (IL-3)
Recombinant human interleukin 6 (IL-6)
Recombinant human erythropoietin (EPO)
Recombinant human granulocyte colony-stimulating factor (G-CSF)
Recombinant human granulocyte-macrophage colony-stimulating factor (GM-CSF)
Supplements

Liquid

Phenol Red