PharmaOnco™ Mouse CD4+CD25+ Treg Cell Isolation Kit

CAT#: ITS-0622-YT425
Product Type: Cell Isolation Kit
Species: Mouse
Target: Non-CD4+ T cells, CD25+ T cells
Description
This kit has been developed to isolate untouched and highly purified mouse CD4+CD25+ Treg cells from single-cell suspensions of splenocytes or other tissues by immunomagnetic negative selection. Isolated cells with up to 92% purity ​are immediately available for downstream applications such as flow cytometry, culture, or cryopreservation.
Applications
Cell Isolation
Application Notes
Desired cells are labeled with antibodies and magnetic particles; Unwanted cells are labeled with antibodies and magnetic particles
Comment
This kit is available for processing 1 x 10^9 cells
Target
Non-CD4+ T cells, CD25+ T cells
Method Type
Negative Selection, Positive Selection
Sample Type
Cells
Research Areas
Immunology, Stem Cell Biology, Cell Therapy Research
Sample Source
Spleen
Size
1 kit
Components
PharmaOnco™ Mouse CD4+ T Cell Isolation Cocktail, 0.5 mL
PharmaOnco™ Streptavidin Magnetic Beads, 1 mL
PharmaOnco™ Mouse CD25+ Treg Cell Positive Selection Cocktail, 0.5 mL
PharmaOnco™ PE Selection Cocktail, 1 mL
PharmaOnco™ Dextran Magnetic Beads, 1 mL
PharmaOnco™ Mouse FcR Blocker, 0.5 mL
PharmaOnco™ Normal Rat Serum, 2 mL
Material not included
Magnetic separator
Cell Background
Regulatory T (Treg) cells
Species
Mouse
Precaution of Use
When handling biohazardous materials such as human cells, safe laboratory procedures should be followed and protective clothing should be worn.
Storage
Store Normal Rat Serum at -20°C; Store other components at 2 - 8°C.
Do not freeze.
Expiry Date
Stable as supplied until expiry date on label.
Note
Tested for sterility
Restrictions
Restrictions for research use only. Intended for use by laboratory professionals.
Shipping
Components may be shipped at room temperature (15 - 25°C). Upon receipt, store it immediately at the temperature recommended below.
For Research Use Only | Not For Clinical Use
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