I-O™ Violet 450 Dye (CAT#: ITS-0622-YT317)

I-O™ Violet 450 Dye is a fixable viability dye which reacts with protein amine groups in both dead and live cells. The dye irreversibly binds free amines available on the cell surface as well as intracellular free amines exposed in cells with compromised cell membranes and can be utilized prior to fixation, permeabilization, or cryopreservation. Due to the compromised cell membrane in dead cells the dye can bind to many more proteins inside the cell, resulting in much higher fluorescence and easy differentation of Live/Dead cells.
I-O™ Violet 450 Dye is excited by the violet laser at 405 nm and it has a fluorescence emission maximum of 450 nm, making it an excellent addition to your multicolor panel.

Flow Cytometry, Immunofluorescence

100 µL

Live/dead mammalian cells

Mammalian cells

1 μl/test

Immunology, Infectious Diseases.

Restrictions for research use only. Intended for use by laboratory professionals.