Immuno-Oncology Cell based Kinase Assay Service

To meet the growing needs in analyzing kinase activity, Creative Biolabs provides a powerful platform to assess the cell-based activity of drug candidates, providing a comprehensive set of critical parameters. We help you streamline your R&D pipeline and obtain high-quality, physiologically relevant data through advanced, non-radioactive high-throughput screening platforms. This allows you to make informed decisions faster and reduce the risk of costly failures in later-stage clinical trials.

Protein Kinases as Important Targets in Cancer

Protein kinases are important targets in drug development, particularly in cancer. More and more kinase inhibitors have been developed successfully for targeting kinases in cancer treatment. To understand how potential kinase inhibitors act on their targets, cell-based kinase assays are suitable. Although great progress in measuring kinase activity with purified proteins has been achieved, questions still exist, such as inaccurate disease models or indirect measurements of drug action. Sensitive and accurate results are highly needed.

For further details on our service offerings and to initiate a project - Request a consultation.

Fig.1 A two-step assay allows determination of absolute AMPK kinase activities.¹

What We Can Offer

Creative Biolabs provides the most comprehensive portfolio of cell-based kinase assays for evaluating kinase activity in the field of drug discovery, including evaluation of kinase activity, assessment of compound binding ability, the measurement of protein interaction, cellular selectivity screening, and compound screening.

Cell-based Kinase Assays at Creative Biolabs

Activity measurement by phosphorylation level or by cell survival is two different cell-based kinase assays that we offer. We offer a large selection of tyrosine kinase targets in cell-based assays, as shown in Table 1. Moreover, we also offer a selection of several serine and Threonine kinase targets, as shown in Table 2. Creative Biolabs has developed a 96-well plate-compatible approach to measure kinase activity, which closes up the gap between single target-focused, high-throughput in vitro assays and lower-throughput cell-based experiments following up.

Table 1. Tyrosine kinase targets in cell-based assays.

Table 2. Serine and Threonine kinase targets in cell-based assays.

The cell kinase phosphorylation assay has been developed to measure kinase activation directly after treating cells with signal pathway activators. Moreover, we have launched the cell tyrosine kinase receptor activity assay to monitor the activity of the major tyrosine kinase receptor family. Our label-free techniques allow the identification of inhibitors that target the ligand-binding domain or kinase domain, which can be used in almost any cancer cell line or primary cell.

Highlights

Expertise in Complex Assays

Creative Biolabs is a leader in complex biological assays. We are committed to providing data that is not only accurate but also clinically meaningful, ensuring you have confidence in your results and research direction.

Bridging the Discovery Gap

Our cell-based assays are designed to bridge the gap between early-stage screening and clinical relevance. By mimicking a natural cellular environment, our assays provide a more accurate predictor of a drug's efficacy and in vivo performance.

Cost-Effective Solutions

Our sophisticated services help you avoid the high costs associated with late-stage drug failures. By identifying issues early on, you can make informed decisions to deprioritize underperforming candidates and focus resources on promising compounds.

Partnership and Support

We offer more than just a service; we are a partner in your research. Our team provides ongoing support, from project initiation to final data interpretation, ensuring your goals are met and your pipeline is streamlined.

Discover how a partnership with us can streamline your research - Get a quote today.

Customer Reviews

• Physiologically Relevant Data
  We faced a common challenge with our traditional biochemical assays; the potency data just didn't translate to our cell-based models. Creative Biolabs' service provided the physiologically relevant data we needed, which directly informed our decision to advance our lead candidate. - K* Chaze*.
• Invaluable Reporting
  The detailed final report we received was invaluable. It provided a clear, visualized comparison of our compound's activity against various kinase targets, something our internal assays couldn't do. The team was also very responsive to our follow-up questions. - M* Thompsn*.

FAQs

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How to Contact Us

Our experienced scientists in kinase detection will provide unparalleled expertise to help you discover kinase drugs. In addition, we also provide a wide range of other options to promote our customer projects forward. Please feel free to contact us.

Reference

1. Yan, Yan et al. "A Highly Sensitive Non-Radioactive Activity Assay for AMP-Activated Protein Kinase (AMPK)." Methods and protocols vol. 1,1 (2018): 3. Distributed under an Open Access license CC BY 4.0, without modification. https://doi.org/10.3390/mps1010003

For Research Use Only | Not For Clinical Use