Immuno-oncology Cell-based Kinase Assay Service

Protein kinases are important targets in drug development, particularly in cancer. More and more kinase inhibitors have been developed successfully for targeting kinases in cancer treatment. To understand how potential kinase inhibitors act on their targets, cell-based kinase assays are suitable. Although great progress in measuring kinase activity with purified proteins has been achieved, questions still exist, such as inaccurate disease models, or indirect measurements of drug action. Sensitive and accurate results are highly needed. To meet the growing needs in analyzing kinase activity, Creative Biolabs provides a powerful platform to assess the cell-based activity of drug candidates, providing a comprehensive set of critical parameters.

What We Offer

Creative Biolabs provides the most comprehensive portfolio of cell-based kinase assays for evaluating kinase activity in the field of drug discovery, including evaluation of kinase activity, assessment of compound binding ability, the measurement of protein interaction, cellular selectivity screening, and compound screening. Our assays include but are not limited to ELISA, AlphaLISA, Western Blot, and MesoScale Discovery (MSD) Assays.

Creative Biolabs provides high-quality kinase testing and kinase screening services to our global customers.

• Biochemical kinase detection
• Screening for kinase inhibitors from large compound libraries
• IC50 determination
• Inhibition study
• Cell kinase and phosphatase pathway detection

Cell-based Kinase Assays at Creative Biolabs

Activity measurement by phosphorylation level or by cell survival is two different cell-based kinase assays we offered. We offer a large selection of tyrosine kinase targets in cell-based assays, as shown in Table 1. Moreover, we also offer selection of several serine and Threonine kinase targets, as shown in Table 2. Creative Biolabs has developed a 96-well plate-compatible approach to measure kinase activity, which closes up the gap between single target-focused, high-throughput in vitro assays and lower-throughput cell-based experiments following up.

Table 1. Tyrosine kinase targets in cell-based assays.

Table 2. Serine and Threonine kinase targets in cell-based assays.

The cell kinase phosphorylation assay has been developed to measure kinase activation directly after treating cells with signal pathway activators. Moreover, we have launched the cell tyrosine kinase receptor activity assay to monitor the activity of the major tyrosine kinase receptor family. Our label-free techniques allow the identification of inhibitors that target the ligand binding domain or kinase domain, which can be used in almost any cancer cell line or primary cell.

Publications Sharing Based on Cell-based Kinase Assay

Assay type: Tyrosine kinase assay

Journal: Journal of biomolecular screening

IF: 3.341

Research Findings: A potent EGF receptor inhibitor from a compound library was identified and characterized by a label-free and real-time cell-based kinase assay. This assay is very easy and simple because it does not require special reagents (such as peptides, antibodies, or probes) and intensive optimization.

Fig.1 Small-molecule inhibitor screen for epidermal growth factor receptor (EGFR) inhibitors using real-time cell electronic sensing.¹

Our experienced scientists in kinase detection will provide unparalleled expertise to help you discover kinase drugs. In addition, we also provide a wide range of other options to promote our customer projects forward. Please feel free to contact us.

Reference

1. Atienza, Josephine M., et al. "Label-free and real-time cell-based kinase assay for screening selective and potent receptor tyrosine kinase inhibitors using microelectronic sensor array." Journal of biomolecular screening 11.6 (2006): 634-643.

For Research Use Only | Not For Clinical Use