PharmaOnco™ 1321N1-Tg(Human P2Y13 Receptor) Division-Arrested Cell, Single Clone

CAT#: ITS-0624-HMM681
Product Type: Division-Arrested Cell
Short Description
Creative Biolabs offers 1321N1-Tg(Human P2Y13 Receptor) Division-Arrested Cell which P2Y13 receptor stably expressed in 1321N1 cells.
Description
1321N1-Tg(Human P2Y13 Receptor) Division-Arrested Cell was engineered to express the receptor human P2Y13 (NM_023914). This cell line can be used to study P2Y13 receptor function, signaling pathways, and potential therapeutic interventions. Dividing-arrest cells are cells that are normally kept under specific culture conditions or treated with agents that prevent cell division from being held in a non-dividing state. This can be achieved through methods such as serum starvation, chemical inhibitors of cell cycle progression, or genetic modification.
Features
Well-characterized stable cell lines;
for cell-based high-throughput screening;
Low-cost evaluation of stable cell lines or limited quantities of compounds.
Applications
P2Y13 receptor function, signaling pathways, and potential therapeutic interventions.
Protein Target
GPCR
Receptor Name
P2Y13
Receptor Family
Purinergic
Species
Human
Parental Cell Line
1321N1
Transfection
Expression vector containing full-length human P2Y13 cDNA (GenBank Accession Number NM_023914) with FLAG tag sequence at N-terminus
Gene
NM_023914
Background
GPR86 or P2Y13 is a 333-amino acid 7 transmembrane protein that lacks a leader peptide but posseses a DRF motif. Northern blot and PCR analysis has shown GPR86 to be expressed highly in the spleen, with weaker expression in placenta, leukocytes, brain, placenta, lung, liver, spinal cord, thymus, small intestine, uterus, stomach, testis, fetal brain and adrenal glad and no expression in the pancreas, heart, kidney, skeletal muscle, ovary or fetal aorta. Communi et al determined that GPR86 shows a high affinity for ADP through pharmacologic charactization of GPR86-transfected human astrocytoma cells and CHO cells.
Unit Size
2 x 10e6 cells/vial, Frozen
Culture Medium
DMEM, 10% FBS
Frozen Formulation
Cell freezing medium
Cell Purity
>95%
Cell Viability
>90%
Sterility Testing
Creative Biolabs provides sterility testing in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.
Identity Testing
Identity testing is required for newly established cell lines. Isoenzyme analysis is used to confirm the identity of the species of a cell line. Alternative methods for identity testing include DNA fingerprinting, STR analysis and karyology.
Virological Safety Testing
A broad range of viruses is susceptible to affecting human cell lines. We can provide in vivo/vitro virus safety assays by utilizing various animal systems. These viruses include: adventitious viruses, bovine viruses, human and simian viruses, porcine viruses, retrovirus and rodent viruses.
Genetic Stability Testing
We perform cell genetic stability studies under ICH guidelines. We can provide guidance on the appropriate testing program upon your requirements.
Mycoplasma Testing
The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.
Shipping
Dry Ice
Storage
Frozen cells should be stored in a liquid nitrogen tank (-150°C~-190°C). After thawing, cells should be immediately transferred to the appropriate culture medium and maintained under recommended culture conditions.
Handling Notes
Frozen cells should be thawed immediately upon receipt and grown according to handling procedure to ensure cell viability and proper assay performance.
Note: Do not freeze the cells upon receipt as it may result in irreversible damage to the cell line.
Disclaimer: We cannot guarantee cell viability if the cells are not thawed immediately upon receipt and grown according to handling procedure.
Warnings
Avoid multiple freeze/thaw cycles
Research Use Only
For research use only, not for diagnostic or therapeutic use.
Quality Control
All of our stable cell lines meet the highest quality standards and have been confirmed to be pathogen-free.
For Research Use Only | Not For Clinical Use
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