PharmaOnco™ 1321N1-Tg(Human P2Y2 Receptor) Division-Arrested Cell, Single Clone

Creative Biolabs offers 1321N1-Tg(Human P2Y2 Receptor) Division-Arrested Cell which P2Y2 receptor stably expressed in 1321N1 cells.

1321N1-Tg(Human P2Y2 Receptor) Division-Arrested Cell was engineered to express the receptor human P2Y2 (NM_002564). This cell line can be used to study P2Y2 receptor function, signaling pathways, and potential therapeutic interventions. Dividing-arrest cells are cells that are normally kept under specific culture conditions or treated with agents that prevent cell division from being held in a non-dividing state. This can be achieved through methods such as serum starvation, chemical inhibitors of cell cycle progression, or genetic modification.

Well-characterized stable cell lines;
for cell-based high-throughput screening;
Low-cost evaluation of stable cell lines or limited quantities of compounds.

P2Y2 receptor function, signaling pathways, and potential therapeutic interventions.

GPCR

P2Y2

Purinergic

Human

1321N1

Expression vector containing full-length human P2Y2 cDNA (GenBank Accession Number NM_002564) with FLAG tag sequence at N-terminus

NM_002564

The P2Y2 receptor was the first of this family of receptors that can be fully activated by both ATP and UTP. P2Y2 receptors are present on all of the body's mucosal surfaces, including the lungs, eyes, upper airways, mouth, vaginal tract and gastrointestinal tract. P2Y2 receptors have also been found on non-mucosal surfaces, such as the retinal pigment epithelium. Activation of P2Y2 has been shown to stimulate mucosal hydration and mucociliary clearance in the lungs and upper airways and induce secretion of therapeutically significant amounts of fluid and tear components to the ocular surface. Thus, P2Y2 receptors are therapeutic targets for treating serious disorders including cystic fibrosis, a fatal genetic disease, retinal detachment as well as dry eye disease.

2 x 10e6 cells/vial, Frozen

DMEM, 10% FBS, 1 μg/mL puromycin

Cell freezing medium

>95%

>90%

Creative Biolabs provides sterility testing in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Identity testing is required for newly established cell lines. Isoenzyme analysis is used to confirm the identity of the species of a cell line. Alternative methods for identity testing include DNA fingerprinting, STR analysis and karyology.

A broad range of viruses is susceptible to affecting human cell lines. We can provide in vivo/vitro virus safety assays by utilizing various animal systems. These viruses include: adventitious viruses, bovine viruses, human and simian viruses, porcine viruses, retrovirus and rodent viruses.

We perform cell genetic stability studies under ICH guidelines. We can provide guidance on the appropriate testing program upon your requirements.

The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.

Dry Ice

Frozen cells should be stored in a liquid nitrogen tank (-150°C~-190°C). After thawing, cells should be immediately transferred to the appropriate culture medium and maintained under recommended culture conditions.

Frozen cells should be thawed immediately upon receipt and grown according to handling procedure to ensure cell viability and proper assay performance.
Note: Do not freeze the cells upon receipt as it may result in irreversible damage to the cell line.
Disclaimer: We cannot guarantee cell viability if the cells are not thawed immediately upon receipt and grown according to handling procedure.

Avoid multiple freeze/thaw cycles

For research use only, not for diagnostic or therapeutic use.

All of our stable cell lines meet the highest quality standards and have been confirmed to be pathogen-free.