PharmaOnco™ CellSort Agarose Column-based Human CD3+ T Cells Selection Kit

This kit has been developed to isolate highly purified human CD3+ T cells from whole blood, buffy coat, peripheral blood mononuclear cells (PBMCs) or other cell suspensions. The CD3 Fab is a recombinantly expressed low affinity Fab fragment. The CD3 Fab-Strep binds to the matrix of Strep-Agarose columns. Therefore CD3+ target cells can adhere to the matrix. Finally, the addition of biotin causes the elution of the target cells from the matrix, and Fab-Streps self-dissociate from the cell surfaces. The label-free authentic target cells are immediately available for downstream applications such as flow cytometry, culture, or cell-based experiments.

Highly pure cells;
Label-free cells

Cell Isolation

Desired cells are labeled with low affinity CD3 Fab fragments.

This kit is available for processing 1 x 10^9 cells.

CD3+ Cell

Positive selection

Cells

Immunology, Stem Cell Biology, Cell Therapy Research

1 kit, 2 tests

CD3+ cells

Human

When handling biohazardous materials such as human cells, safe laboratory procedures should be followed and protective clothing should be worn.

Please place the product at proper storage condition once received.

Store at 2 - 8°C.
Do not freeze.