Description
This kit has been developed to isolate untouched and highly purified naïve CD4+ T cells from mouse lymphoid tissue by negative selection. Isolated cells are immediately available for downstream applications such as flow cytometry, culture, or functional assays.
Features
Fast and easy workflow takes only 30 minutes;
No magnet, no column, no harsh chemicals.
Applications
Cell Isolation
Application Notes
Unwanted cells are labeled with antibodies and deplete.
Comment
This kit is available for processing 1 x 10^9 cells.
Molecular Mechanism of Action
Unwanted cells are labeled with an optimized cocktail of biotinylated antibodies targeting B220 (CD45R), CD11b, CD11c, CD24, CD25, CD44, CD49b, Gr-1and TER-119.
Target
Non-naïve CD4+ T cells
Method Type
Negative selection
Research Areas
Immunology, Stem Cell Biology, Cell Therapy Research
Sample Source
Mouse spleen
Datasheet