PharmaOnco™ CellSort Mouse Naïve CD8+ T Cell (CD3+/CD8+/CD62L+/CD44low) Selection Kit
CAT#: ITS-1222-YT45
Product Type: Cell Isolation Kit
Species: Mouse
Target: Non-naïve CD8+ T cells
Description
This kit has been developed to isolate highly purified Naïve CD8+ T cells from mouse spleen by immunomagnetic negative selection. Non-CD8+ Naïve T cells are depleted by labeling biotin conjugated antibody cocktail mixture followed by incubation with magnetic streptavidin nanobeads. Isolated cells with up to 80-90% purity are immediately available for downstream applications such as flow cytometry, culture, or functional assays.
Applications
Cell Isolation
Application Notes
Unwanted cells are labeled with antibodies and magnetic particles.
Comment
This kit is available for processing 1 x 10^9 cells.
Target
Non-naïve CD8+ T cells
Method Type
Negative selection
Sample Type
Cells
Research Areas
Immunology, Stem Cell Biology, Cell Therapy Research
Sample Source
Other, Spleen
Size
1 kit
Components
PharmaOnco™ Mouse Naïve CD8+ T Cell Biotinylated Antibody Cocktail, 1 mL PharmaOnco™ Buffer, 10 mL PharmaOnco™ Streptavidin Nanoparticles, 1.25 mL
Material not included
Magnetic separator
Cell Background
CD8+ Naïve T cells
Species
Mouse
Precaution of Use
When handling biohazardous materials such as human cells, safe laboratory procedures should be followed and protective clothing should be worn.
Handling Advice
Please place the product at proper storage condition once received.