Description
This kit has been developed to isolate viable IL-10-producing regulatory B cells from single-cell suspensions of lymphoid organs in three steps. Isolated cells are immediately available for downstream applications such as flow cytometry, phenotyping, or functional assays.
Applications
Cell Isolation
Application Notes
Non-B cells are deplete by magnetic labeling; in vitro stimulation; IL-10-secreting B cells are isolated by magnetic labeling.
Comment
This kit is available for processing 2 x 10^9 cells.
Target
Step 1: Non-B cells; Step 3: regulatory B cells.
Method Type
First magnetic separation (negative selection); Next magnetic separation (positive selection)
Research Areas
Immunology, Stem Cell Biology, Cell Therapy Research
Sample Source
Mouse body cavities or spleen
Datasheet