PharmaOnco™ CellSort Three-Step Mouse Reg-B Cell Selection Kit

CAT#: ITS-1222-YT17
Product Type: Cell Isolation Kit
Species: Mouse
Target: Step 1: Non-B cells; Step 3: regulatory B cells.
Description
This kit has been developed to isolate viable IL-10-producing regulatory B cells from single-cell suspensions of lymphoid organs in three steps. Isolated cells are immediately available for downstream applications such as flow cytometry, phenotyping, or functional assays.
Applications
Cell Isolation
Application Notes
Non-B cells are deplete by magnetic labeling; in vitro stimulation; IL-10-secreting B cells are isolated by magnetic labeling.
Comment
This kit is available for processing 2 x 10^9 cells.
Target
Step 1: Non-B cells; Step 3: regulatory B cells.
Method Type
First magnetic separation (negative selection); Next magnetic separation (positive selection)
Sample Type
Cells
Research Areas
Immunology, Stem Cell Biology, Cell Therapy Research
Sample Source
Mouse body cavities or spleen
Size
1 kit, 20 tests
Cell Background
B Cells
Species
Mouse
Precaution of Use
When handling biohazardous materials such as human cells, safe laboratory procedures should be followed and protective clothing should be worn.
Handling Advice
Please place the product at proper storage condition once received.
Storage
Store at 2 - 8°C.
Do not freeze.
For Research Use Only | Not For Clinical Use
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