PharmaOnco™ CellSort Two-Step Human IgM+ Memory B Cell Selection Kit

CAT#: ITS-1222-YT13
Product Type: Cell Isolation Kit
Species: Human
Target: Step 1: Non-B cells which are labeled CD2, CD14, CD16, CD36, CD43, and CD235a (Glycophorin A); Step 2: IgM+ cells.
Description
This kit has been developed to isolate IgM+ memory B cells from human peripheral blood mononuclear cells (PBMCs) by depletion of unwanted cells and subsequent positive selection. Isolated cells ​are immediately available for downstream applications such as flow cytometry, culture, or cell-based experiments.
Applications
Cell Isolation
Application Notes
First step (negative selection): Unwanted cells are labeled with antibodies and deplete; Next step (positive selection): Desired cells are selected with Anti-IgM microbeads.
Comment
This kit is available for processing 2 x 10^9 cells.
Target
Step 1: Non-B cells which are labeled CD2, CD14, CD16, CD36, CD43, and CD235a (Glycophorin A); Step 2: IgM+ cells.
Method Type
Negative selection; positive selection
Sample Type
Cells
Research Areas
Immunology, Stem Cell Biology, Cell Therapy Research
Sample Source
PBMCs
Size
1 kit, 20 tests
Cell Background
B Cells
Species
Human
Precaution of Use
When handling biohazardous materials such as human cells, safe laboratory procedures should be followed and protective clothing should be worn.
Handling Advice
Please place the product at proper storage condition once received.
Storage
Store at 2 - 8°C.
Do not freeze.
For Research Use Only | Not For Clinical Use
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