PharmaOnco™ CHO-Tg(Human PD-L1//TCR Activator) Stable Cell Line, Single Clone

Creative Biolabs offers CHO-Tg(Human PD-L1//TCR Activator) stable cell line which the PD-L1 and TCR Activator gene stably expressed in CHO cells.

The CHO-Tg(Human PD-L1/TCR Activator) stable cell line is a genetically modified Chinese hamster ovary (CHO) cell line that has been engineered to constitutively express both human PD-L1 (Programmed Death-Ligand 1) and a T cell receptor (TCR) activator. PD-L1 is a immune checkpoint protein that inhibits T cell activation, while the TCR activator is designed to stimulate T cell response. This stable cell line can be used in research and drug development to study the interaction between PD-L1 and TCR signaling pathways, as well as to screen potential immunotherapies targeting these pathways.

Consistent gene expression;
Reliability and reproducibility;

Functional assay

PD-L1//TCR Activator

Human

CHO

GenBank accession #NM_014143

The PD-1 gene, also known as programmed cell death protein 1, is a gene that encodes a protein found on the surface of T cells and other immune cells. PD-1 plays a crucial role in regulating the immune response by inhibiting T cell activity and preventing autoimmunity. However, overexpression of PD-1 can also suppress the immune response against cancer cells, allowing tumors to evade detection and destruction by the immune system. Therapies targeting PD-1, such as checkpoint inhibitors, have been developed to block the interaction between PD-1 and its ligands, releasing the brakes on the immune response and helping the body's immune system to attack and eliminate cancer cells. These treatments have shown significant success in treating various types of cancer, including melanoma, lung cancer, and lymphoma.
TCR (T cell receptor) activators are compounds or molecules that stimulate the activation of T cells in the immune system. T cells play a crucial role in the immune response by recognizing and attacking infected cells or foreign invaders. TCR activators can enhance the ability of T cells to detect and eliminate harmful pathogens or cancer cells.

1 x 10e6 cells/vial, Frozen

Creative Biolabs provides sterility testing in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Identity testing is required for newly established cell lines. Isoenzyme analysis is used to confirm the identity of the species of a cell line. Alternative methods for identity testing include DNA fingerprinting, STR analysis and karyology.

A broad range of viruses is susceptible to affecting human cell lines. We can provide in vivo/vitro virus safety assays by utilizing various animal systems. These viruses include: adventitious viruses, bovine viruses, human and simian viruses, porcine viruses, retrovirus and rodent viruses.

We perform cell genetic stability studies under ICH guidelines. We can provide guidance on the appropriate testing program upon your requirements.

The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.

Dry Ice

Frozen cells should be stored in a liquid nitrogen tank (-150°C~-190°C). After thawing, cells should be immediately transferred to the appropriate culture medium and maintained under recommended culture conditions.

Frozen cells should be thawed immediately upon receipt and grown according to handling procedure to ensure cell viability and proper assay performance.
Note: Do not freeze the cells upon receipt as it may result in irreversible damage to the cell line.
Disclaimer: We cannot guarantee cell viability if the cells are not thawed immediately upon receipt and grown according to handling procedure.

Avoid multiple freeze/thaw cycles

For research use only, not for diagnostic or therapeutic use.

All of our stable cell lines meet the highest quality standards and have been confirmed to be pathogen-free.