PharmaOnco™ HEK293-Tg(Human hIDO1) Stable Cell Line, Single Clone

Creative Biolabs offers HEK293-Tg(Human hIDO1) stable cell line which the hIDO1 gene stably expressed in HEK293 cells.

The HEK293-Tg(Human IDO1) stable cell line is a cell line that has been genetically engineered to overexpress human Indoleamine 2,3-dioxygenase 1 (IDO1). IDO1 is an enzyme that plays a key role in regulating the immune response by metabolizing the amino acid tryptophan. This cell line can be used as a valuable tool for studying the mechanisms of IDO1 function and for screening potential drugs that target this enzyme.

Consistent gene expression;
Reliability and reproducibility;

Functional assay

hIDO1

Human

HEK293

Genbank accession number NM_002164

The hIDO1 gene, also known as indoleamine 2,3-dioxygenase 1, codes for an enzyme that catalyzes the first and rate-limiting step in the metabolism of the essential amino acid tryptophan. This enzyme is involved in the degradation of tryptophan along the kynurenine pathway, leading to the production of various metabolites that play important roles in immune regulation and tolerance. The hIDO1 gene has been studied extensively in the context of cancer, autoimmune diseases, and infectious diseases, as its activity can impact immune responses and inflammation. In cancer, overexpression of IDO1 has been linked to tumor immune evasion and poor prognosis, making it a potential target for therapeutic interventions. In autoimmune diseases, dysregulation of IDO1 activity has been implicated in the breakdown of self-tolerance and the development of autoimmune responses. In infectious diseases, IDO1 activity can modulate host immune responses to pathogens.

1 x 10e6 cells/vial, Frozen

Creative Biolabs provides sterility testing in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Identity testing is required for newly established cell lines. Isoenzyme analysis is used to confirm the identity of the species of a cell line. Alternative methods for identity testing include DNA fingerprinting, STR analysis and karyology.

A broad range of viruses is susceptible to affecting human cell lines. We can provide in vivo/vitro virus safety assays by utilizing various animal systems. These viruses include: adventitious viruses, bovine viruses, human and simian viruses, porcine viruses, retrovirus and rodent viruses.

We perform cell genetic stability studies under ICH guidelines. We can provide guidance on the appropriate testing program upon your requirements.

The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.

Dry Ice

Frozen cells should be stored in a liquid nitrogen tank (-150°C~-190°C). After thawing, cells should be immediately transferred to the appropriate culture medium and maintained under recommended culture conditions.

Frozen cells should be thawed immediately upon receipt and grown according to handling procedure to ensure cell viability and proper assay performance.
Note: Do not freeze the cells upon receipt as it may result in irreversible damage to the cell line.
Disclaimer: We cannot guarantee cell viability if the cells are not thawed immediately upon receipt and grown according to handling procedure.

Avoid multiple freeze/thaw cycles

For research use only, not for diagnostic or therapeutic use.

All of our stable cell lines meet the highest quality standards and have been confirmed to be pathogen-free.