PharmaOnco™ HEK293-Tg(Human OX40)-NF-κB Luc Reporter Cell Line, Single Clone

Creative Biolabs offers HEK293-Tg(Human OX40)-NF-κB Luc reporter cell line which the OX40 gene stably expressed in NF-κB Luc reporter HEK293 cells.

HEK293-Tg(Human OX40)-NF-κB Luc reporter cell line is a genetically modified HEK293 cell line that stably expresses the human OX40 receptor and a luciferase reporter gene downstream of the NF-κB promoter. This cell line is commonly used in research to study the activation of the NF-κB signaling pathway in response to OX40 stimulation.

Consistent gene expression;
Reliability and reproducibility;

Functional assay

OX40

Human

HEK293

GenBank Accession #NM_003327

The OX40 gene, also known as TNFRSF4, is a gene that encodes a protein known as OX40 (CD134). OX40 is a member of the tumor necrosis factor receptor superfamily and is primarily expressed on activated T cells. When OX40 binds to its ligand, OX40L, it plays a critical role in regulating T cell activation, proliferation, survival, and cytokine production. The OX40 gene has been implicated in various immune responses, including promoting T cell activation and survival, enhancing memory T cell formation, and regulating the balance between effector and regulatory T cells. Targeting the OX40 pathway has shown promise in cancer immunotherapy, as blocking OX40 signaling can enhance anti-tumor immune responses.

1 x 10e6 cells/vial, Frozen

Creative Biolabs provides sterility testing in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Identity testing is required for newly established cell lines. Isoenzyme analysis is used to confirm the identity of the species of a cell line. Alternative methods for identity testing include DNA fingerprinting, STR analysis and karyology.

A broad range of viruses is susceptible to affecting human cell lines. We can provide in vivo/vitro virus safety assays by utilizing various animal systems. These viruses include: adventitious viruses, bovine viruses, human and simian viruses, porcine viruses, retrovirus and rodent viruses.

We perform cell genetic stability studies under ICH guidelines. We can provide guidance on the appropriate testing program upon your requirements.

The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.

Dry Ice

Frozen cells should be stored in a liquid nitrogen tank (-150°C~-190°C). After thawing, cells should be immediately transferred to the appropriate culture medium and maintained under recommended culture conditions.

Frozen cells should be thawed immediately upon receipt and grown according to handling procedure to ensure cell viability and proper assay performance.
Note: Do not freeze the cells upon receipt as it may result in irreversible damage to the cell line.
Disclaimer: We cannot guarantee cell viability if the cells are not thawed immediately upon receipt and grown according to handling procedure.

Avoid multiple freeze/thaw cycles

For research use only, not for diagnostic or therapeutic use.

All of our stable cell lines meet the highest quality standards and have been confirmed to be pathogen-free.