PharmaOnco™ HEK293-Tg(Human RANK)-NF-κB Luc Reporter Cell Line, Single Clone

Creative Biolabs offers HEK293-Tg(Human RANK)-NF-κB Luc reporter cell line which the RANK gene stably expressed in NF-κB Luc reporter HEK293 cells.

The HEK293-Tg(Human RANK)-NF-κB Luc reporter cell line is a human embryonic kidney cell line that has been engineered to express the human RANK gene and a luciferase reporter gene under the control of the NF-κB pathway. RANK (Receptor Activator of Nuclear Factor κB) is a protein that plays a key role in regulating the NF-κB signaling pathway, which is involved in various cellular processes including inflammation, immune responses, and cell survival. This cell line can be used to study the activation of the NF-κB pathway in response to RANK signaling, as well as to screen for potential drugs or compounds that modulate this pathway.

Consistent gene expression;
Reliability and reproducibility;

Functional assay

RANK

Human

HEK293

GenBank Accession #NM_003839.2

The RANK gene, also known as TNFRSF11A, codes for a protein called Receptor Activator of Nuclear Factor Kappa-B (RANK). RANK plays a crucial role in regulating bone metabolism and is essential for osteoclast differentiation and function. Mutations in the RANK gene have been associated with various bone disorders, such as osteopetrosis and Paget's disease of the bone. The RANK gene is considered to be of high importance in the regulation of bone health and is therefore ranked as a significant gene in the field of bone biology.

1 x 10e6 cells/vial, Frozen

Creative Biolabs provides sterility testing in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Identity testing is required for newly established cell lines. Isoenzyme analysis is used to confirm the identity of the species of a cell line. Alternative methods for identity testing include DNA fingerprinting, STR analysis and karyology.

A broad range of viruses is susceptible to affecting human cell lines. We can provide in vivo/vitro virus safety assays by utilizing various animal systems. These viruses include: adventitious viruses, bovine viruses, human and simian viruses, porcine viruses, retrovirus and rodent viruses.

We perform cell genetic stability studies under ICH guidelines. We can provide guidance on the appropriate testing program upon your requirements.

The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.

Dry Ice

Frozen cells should be stored in a liquid nitrogen tank (-150°C~-190°C). After thawing, cells should be immediately transferred to the appropriate culture medium and maintained under recommended culture conditions.

Frozen cells should be thawed immediately upon receipt and grown according to handling procedure to ensure cell viability and proper assay performance.
Note: Do not freeze the cells upon receipt as it may result in irreversible damage to the cell line.
Disclaimer: We cannot guarantee cell viability if the cells are not thawed immediately upon receipt and grown according to handling procedure.

Avoid multiple freeze/thaw cycles

For research use only, not for diagnostic or therapeutic use.

All of our stable cell lines meet the highest quality standards and have been confirmed to be pathogen-free.