PharmaOnco™ HEK293-Tg(Human TetR) Stable Cell Line, Single Clone

Creative Biolabs offers HEK293-Tg(Human TetR) stable cell line which the TetR gene stably expressed in HEK293 cells.

HEK293-Tg(Human TetR) stable cell line is a type of cell line that has been engineered to express the human tetracycline repressor protein (TetR). TetR is a transcriptional repressor that binds to the tetracycline operator sequence and regulates gene expression. These cells can be used in research to study gene expression and regulation, as well as to induce or repress the expression of specific genes in response to tetracycline or its derivatives.

Consistent gene expression;
Reliability and reproducibility;

Functional assay

TetR

Human

HEK293

The TetR gene is a bacterial gene that encodes for a transcription factor known as Tet repressor. This protein plays a crucial role in regulating the expression of genes that are involved in resistance to tetracycline antibiotics. The TetR protein binds to specific DNA sequences, called operator sequences, and prevents the transcription of genes that confer resistance to tetracycline. In molecular biology and biotechnology, the TetR gene is often used as a tool for gene regulation and expression control. By using TetR-based systems, researchers can turn gene expression on or off in response to the presence or absence of tetracycline or its derivatives.

1 x 10e6 cells/vial, Frozen

Creative Biolabs provides sterility testing in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Identity testing is required for newly established cell lines. Isoenzyme analysis is used to confirm the identity of the species of a cell line. Alternative methods for identity testing include DNA fingerprinting, STR analysis and karyology.

A broad range of viruses is susceptible to affecting human cell lines. We can provide in vivo/vitro virus safety assays by utilizing various animal systems. These viruses include: adventitious viruses, bovine viruses, human and simian viruses, porcine viruses, retrovirus and rodent viruses.

We perform cell genetic stability studies under ICH guidelines. We can provide guidance on the appropriate testing program upon your requirements.

The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.

Dry Ice

Frozen cells should be stored in a liquid nitrogen tank (-150°C~-190°C). After thawing, cells should be immediately transferred to the appropriate culture medium and maintained under recommended culture conditions.

Frozen cells should be thawed immediately upon receipt and grown according to handling procedure to ensure cell viability and proper assay performance.
Note: Do not freeze the cells upon receipt as it may result in irreversible damage to the cell line.
Disclaimer: We cannot guarantee cell viability if the cells are not thawed immediately upon receipt and grown according to handling procedure.

Avoid multiple freeze/thaw cycles

For research use only, not for diagnostic or therapeutic use.

All of our stable cell lines meet the highest quality standards and have been confirmed to be pathogen-free.